金龟子绿僵菌的液固双相发酵研究

金龟子绿僵菌是重要的害虫致病真菌,采用液固双相发酵方法对MA4-37菌株进行发酵,研究液体培养基、固体培养基及其厚度、浅盘固体发酵、袋装固体发酵对金龟子绿僵菌生长及产孢量的影响,优化控制发酵过程。结果表明,液体发酵较适合的培养基为发酵液B(大豆1%、蔗糖1%、NaCl0.5%、MnSO40.5%,用KH2PO4-K2HPO4缓冲液调节pH值为6.0～7.0),采用该培养基时液生分生孢子产量和菌丝生长量均最高,分别达到4.3×109个/L、5.99g/L。固体发酵时,浅盘发酵方法优于袋装发酵,其中采用配方F的固体培养基(碎米600g、大米300g、H2O 50%、KH2PO4-K2HPO4缓冲液0.05%、蔗糖0.5%),厚度在1.3cm时,浅盘培养7d的产粉量可达16.1mg/g。

Liquid-solid Dual-phase Fermentation of Metarhizium anisopliae

Metarhizium anisopliae is an important pest-pathogenic fungus.This experiment carried out liquid-solid dual-phase fermentation of M.anisopliae MA4-37,and researched the effects of liquid medium,solid medium,shallow tray solid fermentation,bagged solid fermentation and solid medium thickness on fungus growth and sporulation,to optimize and control of fermentation process.The screening result showed that liquid culture medium B(soybean 1%,sugar 1%,NaCl 0.5%,and MnSO4 0.5%,with KH2PO4-K2HPO4 buffer adjusting the pH value to 6.0-7.0) was more suitable for liquid fermentation,with which conidia production and mycelial growth amount both reached the highest,4.3×109 conidia/L and 5.99 g/L,respectively.At the stage of solid fermentation,the effect of shallow tray fermentation was better than bagged fermentation.Under shallow tray fermentation,when solid culture medium F(rice 300 g,broken rice 600 g,H2O 50%,KH2PO4-K2HPO4 buffer 0.05%,sucrose 0.5%) with thickness of 1.3 cm was employed,the sporulation capacity(dry spore powder) was up to 16.1 mg/g after 7-day culture.