UCP3基因遗传多态性与中国西门塔尔牛生长

为了分析UCP3基因SNPs与中国西门塔尔牛生长及育肥性状的相关性。以118头中国西门塔尔育肥牛为研究材料，通过DNA测序和PCR-RFLP技术检测UCP3基因BglI酶切位点SNPs，并进行相关分析。UCP3-BglI酶切位点表现多态，且处于Hardy-Weinberg平衡状态。与部分育肥性状的相关分析表明，该位点与饲料转化率性状和平均日增重性状表现显著相关(P<0.05)，其中AA基因型个体均值显著优于AB和BB基因型个体，料肉比较低，日增重较高；与部分生长性状的相关分析发现在15月龄体高、体长和胸围性状，12月龄体高性状，8月龄体长性状中UCP3-BglI多态位点差异显著，AA基因型个体表型值显著高于其他或其中一个基因型值(P<0.05)；在宰后体尺性状测量性状中，UCP3-BglI多态位点对胴体长、胴体胸深和后腿宽和背膘厚性状影响显著。其中AA和AB基因型个体胴体长、胴体胸深和背膘厚性状的表型值显著高于BB基因型个体（P<0.05）；在后腿宽性状中，AB个体表型值显著高于AA和BB个体（P<0.05）。初步认为，AA基因型与优良的生长育肥性状显著相关，对A等位基因的选择有利于优良性状的改良。

Associations of UCP3 Gene Polymorphisms with Growth and Fattening Traits in Simmental

In order to analyze the associations of UCP3 gene polymorphisms with fattening and growth traits in Simmental. The locus of Bgl I of UCP3 gene was genotyped by PCRRFLP and DNA sequencing in Simmental to investigate the associations. The results showed that the locus of Bgl I demonstrated polymorphisms was at HardyWeinberg equilibrium. The associations of polymorphisms of UCP3 gene with fattening traits showed that the individuals with genotype AA had a lower FCR and a higher ADG value （P〈0.05）; As for the growth traits, the locus of Bgl I had an major affect on withers height, body length and chest girth at 15 months of age, withers height at 12 months of age, body length at 8 months of age. Among the phenotypes which were significantly associated, individuals with homozygote of AA genotype had a significantly higher average valuethan that one or the other genotypes （P〈0.05）; About the carcass length, carcass chest depth and fat thickness traits, individuals with homozygote of AA and AB genotype also had a significantly higher average value than that of BB genotype （P〈0.05）. In addition, the individuals with genotype AB had a higher mean value for hind leg wide than those with the genotype AA and BB （P〈0.05）. So the genotype AA was significantly associated with the fine fattening performance and the selection of the allele A was better for the trait improvement.