茉莉酸甲酯对人参β-1,3-葡聚糖酶和几丁质酶活性的影响

为了明确β-1,3-葡聚糖酶和几丁质酶在茉莉酸甲酯（MeJA）诱导人参抗人参锈腐病过程中的作用，试验设4个处理：MeJA、Cylindrocarpon destructans、MeJA+Cylindrocarpon destructans和CK。MeJA浓度为200 mg/L。处理后3，6，9，12，15，20，25，30天测定酶活。结果表明，经MeJA处理后接种人参锈腐菌，人参根系β-1,3-葡聚糖酶和几丁质酶的活性均较对照增强。β-1,3-葡聚糖酶活性在第12天达到峰值，是对照的1.39倍；几丁质酶活性在第15天达到峰值，是对照的1.51倍。这表明β-1,3-葡聚糖酶和几丁质酶在MeJA诱导人参抗人参锈腐病的过程中起重要作用。

Effect of Methyl Jasmonate on the Activities of β-1, 3-glucanase and Chitinase in Ginseng

The aim was to study the function of β-1,3-glucanase and chitinase in ginseng resistance to Cylindrocarpon destructans induced by Methyl Jasmonate.Four treatments were designed in the test,including MeJA,Cylindrocarpon destructans,MeJA ＋ Cylindrocarpon destructans and CK.Ginseng plants were immersed in Methyl Jasmonates solution（200 mg/L）.The ginseng plants were obtained after 3,6,9,12,15,20,25,30 days.The activities of β-1,3-glucanase and chitinase were measured.The results showed that,both the activities of β-1,3-glucanase and chitinase could be increased significantly in the ginseng roots induced by MeJA than those in control.The activities of β-1,3-glucanase reached the peak at 12 days after inoculation,of which was 1.39 times of CK;and chitinase activity reached the peak at 15 days after inoculation,was 1.51 times of CK.The findings indicated that β-1,3-glucanase and chitinase play critical roles in resistance to C.destructans in ginseng induced by Methyl Jasmonate.