葡萄炭疽病菌SRAP遗传多样性分析

探讨葡萄炭疽病菌的变异和群体结构,为进一步深入研究葡萄炭疽病的发生、流行及防控技术提供理论依据。采用SRAP分子标记技术对不同地区的25个葡萄炭疽菌(Colletotrichum gloeosporioides)菌株进行遗传多样性分析。利用4个菌株从100对引物组合中筛选出6对扩增条带多样性丰富、稳定性较好的引物组合;对供试的25个菌株进行SRAP扩增,共得到164条清晰可辨的条带,其中多态性条带为156条,多态性比率为95.12%;利用NTSYS-2.1软件进行病原菌的聚类分析,其相似性系数在0.61~0.95之间。不同地区葡萄炭疽菌的亲缘关系较近,遗传多样性丰富,但各菌株间存在遗传差异,且菌株之间的差异与地理来源无明显相关性。

Analysis on Genetic Diversity of Colletotrichum gloeosporioides Based on SRAP

In order to provide a theoretical basis for the occurrence, epidemic and control technologies of the disease, the variation and genetic structure of Colletotrichum gloeosporioides were explored. Sequence related amplified polymorphism （SRAP） analysis was used to detect the genetic variation in populations of the 25 C. gloeosporioides in different areas. Among 100 combinations of primer pairs screened, 6 primer pairs that amplified consistent polymorphic bands with DNA of 4 isolates were generated. They were further applied to amplify the genomic DNA of the 25 tested isolates. A total of 164 discernible bands were obtained, 156 of which showed polymorphism, and similarity coefficient was 95.12%. The dendrogram was constructed with the software NTSYSpe Version2.1 and the similarity coefficients were ranged from 0.61-0.95. The result indicated there was a close genetic relationship and abundant genetic diversity. Nonetheless, rich genetic variation existed among the tested isolates and there was no correlation between SRAP group and the geographic origin.