Identification of genes expressed during spore germination of Mycosphaerella pinodes |
| |
| Authors: | Hiroyuki Takahara Kazuhiro Toyoda Gento Tsuji Yasuyuki Kubo Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi |
| |
| Affiliation: | (1) Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, Okayama, Japan;(2) Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan |
| |
| Abstract: | ![]()
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. |
| |
| Keywords: | Mycosphaerella pinodes Pisum sativum Pycnospore germination RT-PCR Suppression subtractive hybridization (SSH) |
| 本文献已被 SpringerLink 等数据库收录！ |
|
