| 合成脂肪酶的影响 |
| |
| 引用本文： | 勇强,姜镇河,余世袁.合成脂肪酶的影响[J].林产化学与工业,2004,24(4):1-6. |
| |
| 作者姓名： | 勇强 姜镇河 余世袁 |
| |
| 作者单位： | 1. 南京林业大学,化学工程学院,江苏,南京,210037
2. 韩国全北大学校,农科大学,全州,561-765 |
| |
| 基金项目： | 国家自然科学基金资助项目 (3 0 1 0 0 1 42 ) |
| |
| 摘 要： | 研究了碳源和氮源对白腐菌Sarcodon asparatus合成脂肪酶的影响。橄榄油是脂肪酶合成的最佳碳源，脂肪酶活力达0．65U／mL。蛋白胨、尿素和氯化铵对脂肪酶的合成有促进作用，它们的酶活力分别为0．65、0．67和0．62U／mL，而硫酸铵抑制脂肪酶的合成，其酶活力仅为0．08U／mL。C／N比对细胞生长和酶的合成影响很大，当C／N比从2增加到10时，脂肪酶活力从0．65下降到0．14U／mL，而细胞浓度从3．08升高到4．58mg／mL。在培养基初始pH值5．5、28℃下培养，S．asparatus合成的脂肪酶活力最高，脂肪酶活力和细胞浓度分别为0．65U／mL和3．08mg／mL。
|
| 关 键 词： | 脂肪酶 碳源 氮源 白腐菌 Sarcodon asparatus |
EFFECTS OF CARBON AND NITROGEN SOURCES ON LIPASE PRODUCTION BY SARCODON ASPARATUS |
| |
| Abstract.EFFECTS OF CARBON AND NITROGEN SOURCES ON LIPASE PRODUCTION BY SARCODON ASPARATUS[J].Chemistry & Industry of Forest Products,2004,24(4):1-6. |
| |
| Authors: | Abstract |
| |
| Abstract: | Carbon sources and nitrogen sources affecting lipase production by Sarcodon asparatus were investigated.Lipase activity was the highest(0.65 U/mL) for olive oil compared with other carbon sources.Lipase production was stimulated by peptone(0.65 U/mL),urea(0.67 U/mL) and ammonium chloride(0.62 U/mL),and repressed by ammonium sulfate with a low lipase activity of 0.08 U/mL.C/N ratio was a significant factor for lipase production and cell growth.When C/N ratio was raised from 2 to 10,lipase activity decreased from 0.65 to 0.14 U/mL,and biomass increased from 3.08 to 4.58 mg/mL.The optimal culture conditions of S.asparatus were pH value 5.5 and 28 ℃,and lipase activity was 0.65 U/mL with a biomass concentration of 3.08 mg/mL. |
| |
| Keywords: | lipase carbon source nitrogen source white rot fungi Sarcodon asparatus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《林产化学与工业》浏览原始摘要信息 |
