| 抗除草剂基因在黄瓜杂种纯度快速鉴定上的应用研究 |
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| 引用本文： | 金红,杜胜利,陈峥,张力,程奕,魏爱民,王永.抗除草剂基因在黄瓜杂种纯度快速鉴定上的应用研究[J].华北农学报,2004,19(3):31-34. |
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| 作者姓名： | 金红 杜胜利 陈峥 张力 程奕 魏爱民 王永 |
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| 作者单位： | 天津市农业科学院中心实验室,天津,300192;天津科润黄瓜研究所,天津,300192 |
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| 基金项目： | 天津市自然科学基金(003802611) |
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| 摘 要： | 黄瓜抗除草剂转基因T0植株经除草剂抗性筛选，连续2代自交获得T1和T2种子；分别对T1植株进行bar基因PCR检测和T2植株Southern杂交检测，证明bar基因已整合到黄瓜染色体上；以非转基因黄瓜为对照，摸索出田间抗性鉴定和室内种子抗性鉴定的除草剂临界浓度；从田间和室内筛选除草剂抗性纯合性转基因株系3个；以表现较好的2个抗性纯合转基因株系为父本，与非转基因母本杂交，获得F1种子，并在室内进行了杂交种纯度鉴定，鉴定效率达100％。建立了一套在种子发芽阶段或2片真叶期进行黄瓜杂交种纯度鉴定的新技术。
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| 关 键 词： | bar基因 转基因 黄瓜 杂交种子 纯度检测 纯合性 |
| 文章编号： | 1000-7091(2004)03-0031-04 |
Application Studies on Quick Testing of Purity of Cucumber Hybrid Seeds by Anti-herbicide Gene |
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| JIN Hong,DU Sheng-li,CHEN Zheng,ZHANG Li,CHENG Yi,WEI Ai-min,WANG Yong.Application Studies on Quick Testing of Purity of Cucumber Hybrid Seeds by Anti-herbicide Gene[J].Acta Agriculturae Boreali-Sinica,2004,19(3):31-34. |
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| Authors: | JIN Hong DU Sheng-li CHEN Zheng ZHANG Li CHENG Yi WEI Ai-min WANG Yong |
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| Abstract: | Seeds of T1 and T2 were obtained from T0 cucumber anti-herbicide transgenic plants selected by herbicide resistance and self-cross. Bar gene was showed to be integrated into cucumber chromosomes. Threshold concentration of herbicide used as field detection and lab detection were selected; 3 transgenic homozygosis strains in herbicide resistance were obtained, two of which were used as male parent to hybrid with non-transgenic female parent and get F1 hybrid seeds; test method of purity of hybrid seeds was established in lab and the test was finished during shoots and two leaves seedlings. . |
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| Keywords: | Bar gene Transgenic Cucumber Hybrid seeds Test purity Homozygosis |
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