甘蓝与萝卜属间杂交及其胚挽救技术优化

以甘蓝显性雄性不育系C08-11为母本，以萝卜自交系Q07-12为父本进行属间杂交，对杂交子房和胚珠进行离体培养，筛选适合甘蓝与萝卜属间杂种胚挽救的适宜取材时间、培养基及外源添加物。结果表明，胚珠培养优于子房培养；所有处理子房培养仅获得3株杂交苗，取材时间以授粉后第9天为宜，B5培养基中添加合适的生长调节剂、水解酪蛋白和活性炭为子房培养所必需；胚珠培养各处理共获得29株杂交苗，适宜取材时间为蕾期授粉后第14天、花期授粉后第16天，对生长调节剂的需求取决于不同取材时期及培养基。

Optimization of Technology for Embryo Rescue in Distant Hybridization between Cabbage and Radish

The distant hybridization between dominant male sterile line of cabbage‘C08-11’and radish inbred line‘Q07-12’was made in this experiment.Through in vitro culture of cross-bred ovary and ovule at different developmental stages,the appropriate sampling time，culture medium and additives for the embryo rescue in distant hybridization between cabbage and radish were studied.The results were as follows:ovule culture was obviously better than ovary culture.By ovary culture only 3 hybrid seedlings were gotten and the suitable time for detaching ovary was 9 days after pollination.Adding growth regulators（6-BA，NAA）,casein acids hydrolysate and activated charcoal in basic medium B5 was necessary for ovary culture.By ovule culture，29 hybrid seedlings were gotten and the appropriate time for detaching ovule was respectively 14 days after bud pollination and 16 days after florescence pollination.Whether adding growth regulator or not depended on the sampling period and culture medium for ovule culture.