Biochemical Evidences for Scopoletin Inhibits Ca~(2+)-ATPase Activity in the Carmine Spider Mite,Tetranychus cinnabarinus(Boisduval)

[Objective] This study aimed to investigate the acaricidal effect of scopoletin, and provide the biochemical evidences of scopoletin influences Ca2 +-ATPase activity and gene expressions in the Carmine Spider Mite, Tetranychus cinnabarinus.[Method] The acaricidal effects of scopoletin were investigated by slip-dip method.Exposeed to different concentrations of scopoletin(0.16-2.5 mg/ml), Ca2+-ATPase activity in vivo and protein contents were investigated. For assessing the in vitro effect, Ca2+-ATPase enzyme(200 μl) prepared from normal mites were incubated with different concentrations of scopoletin reagents. [Result] Scopoletin exhibited significant inhibitory effect on Ca2+-ATPase activity both in vivo and in vitro, and resulted in increased protein contents; kinetic analysis showed that the catalytic capability of Ca2+-ATPase was significantly reduced by scopoletin. [Conclusion] Scopoletin exhibits a significant inhibitory effect on Ca2 +- ATPase, and its acaricidal effect against T. cinnabarinus might be due to the direct inhibition of Ca2+-ATPase.