| 里氏木霉外源表达载体的构建 |
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| 引用本文： | 张晓烜,王傲雪.里氏木霉外源表达载体的构建[J].安徽农业科学,2011,39(13):7617-7620. |
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| 作者姓名： | 张晓烜 王傲雪 |
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| 作者单位： | 东北农业大学成栋学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 目的]构建里氏木霉外源表达载体。方法]以里氏木霉40359的CBHI启动子和终止子构建了里氏木霉40359外源表达载体,并用该表达载体成功表达了抗潮霉素B磷酸转移酶基因,得到6株可在含175 mg/L潮霉素的基础培养基上生长的抗性菌株;提取6株抗性菌株的DNA整合到里氏木霉菌株中,并对其进行潮霉素耐受性检测。结果]6株抗性菌株的抗潮霉素能力比原始菌株提高了75%;转基因菌株的潮霉素抗性可以稳定遗传,证明表达载体构建成功。结论]为开展里氏木霉分子生物学研究与遗传改造奠定了基础。
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| 关 键 词： | 里氏木霉 载体 遗传转化 |
Establishment of Trichoderma reesei Construct for Expressing External Genes |
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| ZHANG Xiao-xuan et al.Establishment of Trichoderma reesei Construct for Expressing External Genes[J].Journal of Anhui Agricultural Sciences,2011,39(13):7617-7620. |
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| Authors: | ZHANG Xiao-xuan |
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| Institution: | ZHANG Xiao-xuan et al(Chengdong College,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | Objective] The study was to construct a Trichoderma reesei vector for expressing external genes.Method] Using CBHI promoter and terminator of T.reesei strain 40359,we constructed an expression vector of T.reesei strain 40359 for expressing Hpt gene and got 6 strains capable of growing on basic medium containing 175 mg/L hygromycin B,further conducted hygromycin resistance test.Result] In comparison with the original strain(wild type),hygromycin resistance the 6 engineered strains was increased by 75%;the... |
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| Keywords: | Trichoderma reesei Vector Genetic transformation |
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