番红花花芽分化期显微结构及核酸、可溶性蛋白质量分数的变化

运用扫描电镜技术观察番红花(Crocus sativus L.)花芽不同分化期的形态变化,测定不同分化及发育期的核酸和可溶性蛋白质量分数,并分析了核酸与可溶性蛋白质量分数变化的相关性。结果表明:番红花花器官按照萼片—外轮花被—雄蕊—内轮花被—雌蕊的顺序分化,蝎尾状聚伞花序,从花芽分化开始到花器官功能形成共经历65 d左右;番红花花芽分化过程中,RNA质量分数和RNA质量分数/DNA质量分数先升高后降低,DAN、可溶性蛋白质量分数均呈现"升-降-升"的变化趋势,核酸与可溶性蛋白质量分数之间存在显著或极显著相关性。由此说明,番红花属于典型的ABC植物,核酸和蛋白质代谢共同调节番红花花芽的分化和发育,是营养生长过渡为生殖生长及花芽形态建成的生化基础。

Contents of Microscopic Structure,Nucleic Acid and Soluble Protein during Flower Bud Differentiation in Crocus sativus

Scanning electron microscope was applied to observe the formal change of Crocus sativus L.during its different flower bud differentiation stages , measure the contents of nuclein acid and soluble protein in differential growth periods , and study the co-relation between them .The differentiation sequence of its flower organ as follows:sepals outer tepals stamens inner tepals pistils, as for scorpioid cyme , it took about 65 d from the beginning of flower bud differentiation to the formation of floral organ.During the flower bud differentiation process , the contents of RNA/DNA and RNA were increased firstly , and then decreased, and those of DNA and soluble protein presented a “up-down-up” trend, and there was a significant or highly significant correlation between the contents of nucleic acid and soluble protein .Thus, C.sativus L was a typical ABC plant, and nucleic acid and protein metabolism commonly regulated the differentiation and development of flower bud , and was the biochemical basis of the transition from vegetative growth to reproductive growth and flower bud morphogenesis .