阿替美唑对小型猪特异性麻醉剂诱导大鼠大脑皮质内Fos蛋白表达的影响

研究阿替美唑对小型猪特异性麻醉剂(XFM)麻醉下大鼠大脑皮质Fos蛋白表达的影响,探讨阿替美唑颉颃XFM催醒大鼠与大脑皮质c-fos基因的关系。将72只SD纯种大鼠随机分为XFM对照组、XFM+阿替美唑组、XFM+生理盐水组,每组又按采脑时间点分成4个亚组。采用蛋白质印迹法检测大脑皮质内Fos蛋白表达量。结果表明:XFM麻醉大鼠大脑皮质内Fos蛋白表达量逐渐增加,与给药后10min比较差异显著(P<0.01或P<0.05);XFM麻醉大鼠腹腔注射生理盐水,各时间点Fos蛋白表达量与对照组间比较无显著差异(P>0.05);阿替美唑注射后引起XFM麻醉大鼠大脑皮质Fos蛋白表达量减少,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果提示,大脑皮质c-fos基因参与了阿替美唑颉颃XFM麻醉作用。阿替美唑抑制XFM诱导大鼠大脑皮质Fos蛋白表达,可能是阿替美唑催醒XFM麻醉大鼠的重要机理之一。

Effect of Atipamezole on the Expression of Fos Protein Induced by Specificity cAnesthetic for Miniature Pigs in the Rat Cerebral Cortex

The research was conducted to investigate relationship atipamezole woke up rat anesthetized by XFM to c-fos oncogene,by study on effect of atipamezole on the expression of Fos protein induced by XFM in the rat cerebral cortex.72 SD rats were divided randomly into XFM group,XFM add atipamezole group,XFM add saline group.The content of c-fos protein in cerebral cortex was measured by western blot.The Fos protein contents were obviously increased in the XFM anesthesia group as compared with 10 min after XFM administrated(P<0.01 or P<0.05).The Fos protein contents was never obviously changed after saline administrated as compared with control group(P>0.05).The Fos protein contents was significantly decreased after atipamzole administrated as compared with control group(P<0.05 or P<0.01).These results indicated that atipamezole palinesthesia action was concerned with c-fos oncogene in cerebral cortex.Atipamzole attenuates expression of Fos protein induced by XFM in the rat cerebral cortex,it may be an important mechanism of atipamzole wake up rat anaesthetized by XFM.