1株氟氯氰菊酯降解菌GZ-3的分离和鉴定

[目的]研究氟氯氰菊酯降解菌GZ-3的分类鉴定和降解性能。[方法]通过富集筛选的方法,从农药污染的土壤中筛选到1株氟氯氰菊酯的高效降解菌GZ-3,观察其菌落及菌体形态特征,通过培养观测其对氟氯氰菊酯的降解率,并对其进行16S rDNA同源性序列分析和生理生化特征分析,还利用Biolog生态板就氟氯氰菊酯降解菌对31种碳源利用情况进行初步研究。[结果]氟氯氰菊酯降解菌GZ-3在37℃和220r/min培养条件下培养72h后对初始浓度为50mg/L的氟氯氰菊酯的降解效率可达80%以上。同源性分析结果表明,该菌株的16S rDNA序列与多数铜绿假单胞菌的序列同源性均在99%以上,结合生理生化特性,初步鉴定该菌株属于铜绿假单胞菌。[结论]该研究为进一步利用该菌对氟氯氢菊酯农药污染的治理奠定基础。

Isolation and Identification of A Cyfluthrin-degradation Bacteria GZ-3

[Objective]The study aimed to research the classification identification and the degradation performance of cyfluthrin-degrading bacteria GZ-3. [Method]A high-efficiency cyfluthrin-degrading bacteria GZ-3 was screened from pesticide-contaminated soil through the enrichment and screening method,its the bacterial colony morphology were observed,its degradation rate on the cypermethrin was observed and its 16S rDNA sequencing homology analysis and the physiological and biochemical characteristics analysis were made out. The utilization status of cyfluthrin-degrading bacteria on 31 kinds of carbon source was studied preliminarily by using Biolog eco-boards.[Result]Cyfluthrin-degrading bacterium GZ-3 showed the degradation rate of over 80% on the cypermethrin at the initial concentration of 50 mg/L under the culture conditions at 37 ℃ and 220 r/min for 72 h. Homology analysis showed that the 16S rDNA sequences of the strain had the sequence homology of more than 99%with the majority of Pseudomonas aeruginosa,and combined with its physiological and biochemical characteristics,it was identified preliminarily that the strain belonged to P. aeruginosa. [Conclusion]The study laidA the foundation for the governance of HCFC pyrethroid pesticide contamination by further using the bacteria.