| 链球菌表面烯醇化酶重组蛋白多克隆抗体的制备 |
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| 引用本文： | 许丽萍,白文成,刘杨. 链球菌表面烯醇化酶重组蛋白多克隆抗体的制备[J]. 内蒙古农业大学学报(自然科学版), 2012, 33(1): 20-23 |
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| 作者姓名： | 许丽萍 白文成 刘杨 |
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| 作者单位： | 内蒙古医学院基础医学院;内蒙古农业大学兽医学院;血浆脂蛋白免疫学研究中心;内蒙古农业大学生物工程学院 |
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| 基金项目： | 国家自然科学基金(30860019)资助 |
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| 摘 要： | 制备M6型GAS表面烯醇化酶抗体。皮下多点注射GAS表面烯醇化酶重组蛋白(rSEN)免疫家兔,采用亲和层析纯化免疫血清,通过SDS-PAGE、ELISA、Western-Blot等方法检测抗体的特异性,并初步用制备的抗体检测了M6血清型GAS表面烯醇化酶的表达。制备的抗体蛋白纯度较高,检测的特异性较强,用制备的抗体可以检测到活体菌表面的烯醇化酶。成功制备并纯化得到了GAS表面烯醇化酶多克隆抗体。
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| 关 键 词： | A群链球菌 表面烯醇化酶 多克隆抗体 |
Preparation of Recombinant Streptococcal Surface Enolase Polyclonal Antibody |
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| XU Li-ping , BAI Wen-cheng , LIU Yang. Preparation of Recombinant Streptococcal Surface Enolase Polyclonal Antibody[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2012, 33(1): 20-23 |
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| Authors: | XU Li-ping BAI Wen-cheng LIU Yang |
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| Affiliation: | 2,4(1.College of Basic Medicin of Inner Mongolia Medical University,Hohhot 010059,China,Inner Mongolia Agricultural University; 2.College of Veterinary Medicine,Hohhot 010018,China;3.College of Life Sciences,Hohhot 010018,China; 4.Research Center of Plasma Lipoprotein Immunology 010018,China) |
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| Abstract: | To prepare anti-rSEN antibody and to detect the express of surface enolase of GAS.Rabbit was administered three times with rSEN subcutaneously by mutli-dot,pured immune serum by affinity chromatograph and detected the specification of anti-rSEN polyclonal antibody with SDS-PAGE,Western-Blot and ELISA.The expression of surface enolase of serotype M6 of GAS also was determined with anti-rSEN antibody.Prepared anti-rSEN polyclonal antibody was profuse and specification is obvious.The purified anti-rSEN antibody can detect the express of surface enolase of GAS.Anti-rSEN polyclonal antibody was succusfully prepared and purified. |
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| Keywords: | Group A streptococcus surface enolase polyclonal antibody |
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