| 蒙古黄榆愈伤诱导及植株再生的初步研究 |
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| 引用本文： | 图亚,叶冬梅,张文泉,白玉娥.蒙古黄榆愈伤诱导及植株再生的初步研究[J].内蒙古农业大学学报(自然科学版),2012,33(1):38-42. |
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| 作者姓名： | 图亚 叶冬梅 张文泉 白玉娥 |
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| 作者单位： | 内蒙古农业大学林学院,呼和浩特,010019 |
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| 基金项目： | 内蒙古自然科学基金，内蒙古自治区高等学校科学研究项目 |
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| 摘 要： | 以蒙古黄榆无菌苗叶片与茎段为外植体,研究了不同生长调节剂组合对蒙古黄榆愈伤组织诱导及分化的影响,并获得了完整的再生植株.结果表明:最佳外植体为无菌苗叶片,诱导愈伤组织的最佳培养基组合为MS +2,4-D 1.5mg/L+6- BA 0.2mg/L；愈伤组织分化的最佳培养基组合为MS+IAA 5.0 mg/L+6-BA 1.0mg/L；诱导生根的最佳培养基组合为1/2MS+1.0 mg/L IBA.
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| 关 键 词： | 蒙古黄榆 组织培养 植株再生 |
STUDIES ON THE CALLIFEROUS INDUCTION AND PLANT REGENERATION OF Ulmus macrocarpa VAR.Mongolica |
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| TU Ya
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YE Dong-mei
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ZHANG Wen-quan
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BAI Yu-e.STUDIES ON THE CALLIFEROUS INDUCTION AND PLANT REGENERATION OF Ulmus macrocarpa VAR.Mongolica[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(1):38-42. |
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| Authors: | TU Ya YE Dong-mei ZHANG Wen-quan BAI Yu-e |
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| Institution: | (Forestry College,Inner Mongolia Agricultural University,Huhhot 010019) |
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| Abstract: | The effect of hormone on the callus induction and differentiation of leaves and stem of Ulmus macrocarpa var.mongolica was studied.And an efficiency regeneration System of the plant was established.The results showed:leaves was the best explants for the tissue culture,the optimum medium for callus induction from leaves was MS+ 1.5mg/L 2,4-D+0.2mg/L6-BA.The optimum medium for callus differentiation was MS+ 5.0mg/LIAA+ 1.0mg/L6-BA;and the adventitious root was induced on 1/2MS+1.0 mg/L IBA. |
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| Keywords: | Ulmus macrocarpa var mongolica Tissue cultur plant regeneration |
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