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| 龙胆提取物高效液相色谱指纹图谱研究 | |
| 引用本文： | 姜辉,王艳艳,孙佳明,王英平. 龙胆提取物高效液相色谱指纹图谱研究[J]. 特产研究, 2011, 33(2): 22-24,46 |
| 作者姓名： | 姜辉 王艳艳 孙佳明 王英平 |
| 作者单位： | 1. 吉林特研药业有限公司,吉林吉林,132109 2. 河南工业大学,郑州450052;中国农业科学院特产研究所,吉林吉林132109 3. 长春中医药大学中医药与生物工程研发中心,长春,130117 4. 中国农业科学院特产研究所,吉林吉林,132109 |
| 基金项目： | 国家科技部支撑计划项目 |
| 摘 要： | 建立龙胆提取物的高效液相色谱指纹图谱。以龙胆提取物为研究对象,应用反相高效液相色谱法,色谱柱为Hypersil ODS（4.6mm×250mm,5μm）,甲醇-水二元梯度洗脱模式,流速为1.0m/min,检测波长为270nm,建立了10批不同批次的龙胆提取物的HPLC指纹图谱。应用＂中药色谱指纹图谱相似度评价系统＂（2004A版）对各批次龙胆提取物进行了质量评价。该方法简便、可靠,可用于龙胆提取物的真伪鉴别和质量评价。 |
| 关 键 词： | 龙胆提取物 指纹图谱 反相高效液相色谱法 |
Studies on HPLC Fingerprint of Gentianae Radix et Rhizoma |
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| JIANG Hui,WANG Yan-yan,SUN Jia-ming,WANG Ying-ping. Studies on HPLC Fingerprint of Gentianae Radix et Rhizoma[J]. Special Wild Economic Animal and Plant Research, 2011, 33(2): 22-24,46 | |
| Authors: | JIANG Hui WANG Yan-yan SUN Jia-ming WANG Ying-ping |
| Affiliation: | JIANG Hui1,WANG Yan-yan2,3,SUN Jia-ming4,WANG Ying-ping3(1.Jilin Teyan Pharmaceutical Co.,Ltd.,Jilin 132109,China,2.China.Henan University of Technology,Zhengzhou 450052,3.Institute of Special Wild Economic Animals and Plants,CAAS.Jilin 132109,4.Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Traditional Chinese Medicine,Changchun 130117,China) |
| Abstract: | To establish the RP-HPLC fingerprint analysis for the quality control of Gentianae Radix et Rhizoma.HPLC fingerprint analy-sis method for Gentianae Radix et Rhizoma was developed,Hypersil ODS5μm(4.6 mm×250 mm) was used,with mixture of methanol and water as mobile phase in a gradient mode.The flow rate was 1.0 mL/min.The wavelength of measurement was 270 nm.The samples’ quality was assessed by Similarity Evaluation System for Chromato-graphic Finger print of TCM2004.The method can be used to identify and evaluate the quality Gentianae Radix et Rhizoma. |
| Keywords: | Gentianae Radix et Rhizoma Chromatographic fingerprint RP-HPLC |
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