| 猫瘟热病毒胶体金快速检测试纸的研究 |
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| 引用本文： | 燕永彬,孙静,艾有为,田丽红.猫瘟热病毒胶体金快速检测试纸的研究[J].经济动物学报,2011,15(2):82-84,87. |
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| 作者姓名： | 燕永彬 孙静 艾有为 田丽红 |
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| 作者单位： | 东北林业大学野生动物资源学院,哈尔滨,150040 |
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| 基金项目： | 东北林业大学大学生创新实验项目(101022511); 国家林业局野生动植物保护管理项目(2011) |
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| 摘 要： | 利用双抗体夹心和胶体金免疫层析技术的原理,在玻璃纤维膜、硝酸纤维膜的检测线(T)和对照线(C)上分别喷上胶体金与猫瘟热病毒(FPV)单克隆抗体(3C4)的偶联物、FPV多克隆抗体和羊抗鼠IgG单克隆抗体,制成检测FPV抗原的猫瘟热病毒胶体金快速检测试纸,对临床26份虎粪便样本进行检测,并与PCR检测法及韩国(ASAN ...
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| 关 键 词： | 猫瘟热病毒 单克隆抗体 胶体金 免疫层析 |
Colloidal Gold Strip for Detection of Feline Panleukopenia Virus |
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| YAN Yong-bin,SUN Jing,AI You-wei,TIAN Li-hong.Colloidal Gold Strip for Detection of Feline Panleukopenia Virus[J].Journal of Economic Animal,2011,15(2):82-84,87. |
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| Authors: | YAN Yong-bin SUN Jing AI You-wei TIAN Li-hong |
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| Institution: | YAN Yong-bin,SUN Jing,AI You-wei,TIAN Li-hong(College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | Based on the principle of colloidal gold labeling technique and double antibody sandwich immunoassay,the coupling product from colloidal gold and FPV McAb(3C4) were sprinkled on the glassfiber film.FPV polyclonal antibody and monoclonal antibodies(goat anti-mouse IgG) were sprinkled on the detection line and control line in nitrocellulose membrane,colloidal gold strip was executed to detect FPV antigen quickly.The contrast detection of the 26 faeces samples of tigers showed that the detection rate by colloi... |
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| Keywords: | feline panleukopenia virus monoclonal antibodies colloidal gold immunochromatography assay |
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