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| 蒙古马与其他品种马的ELA-DRA*exon2多态性分析 | |
| 引用本文： | 李金莲,石有斐,刘淑娟,芒来.蒙古马与其他品种马的ELA-DRA*exon2多态性分析[J].山东农业大学学报(自然科学版),2007,38(3):345-350. |
| 作者姓名： | 李金莲 石有斐 刘淑娟 芒来 |
| 作者单位： | 1. 泰山学院生物科学与技术系,山东,泰安,271021 2. 山东农业大学动物科技学院,山东,泰安,271018 3. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 |
| 基金项目： | 泰山学院校科研和教改项目;内蒙古高等学校科研项目 |
| 摘 要： | 本实验通过PCR-SSCP技术检测了三大品种6个类型共296匹马ELA-DRA* exon2的多态性。用12%非变性聚丙烯酰胺凝胶电泳将已变性的PCR扩增产物分离,并用银染法显色。结果296匹马中共出现6种基因型:三种纯合子分别记为AA、BB和CC型,均为3条带;三种杂合子分别记为AB、BC和AC型,均为4条带;且AA和AB两种基因型是所研究的各类型马的优势基因型。纯血马和三河马的PIC和He值均较高,属于高度多态,4个类型蒙古马均属于中度多态。聚类分析表明三大品种各聚一支,各类型蒙古马中锡尼河马和巴尔虎马关系较近。 |
| 关 键 词： | 马 多态性 |
| 文章编号： | 1000-2324（2007）03-0345-06 |
| 修稿时间： | 2006-06-17 |
ANALYZED OF ELA-DRA*EXON2 POLYMORPHISM BETWEEN MONGOLIAN HORSE AND OTHER HOURSE BREEDS |
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| LI Jin-lian,SHI Yon-fei,LIU Shu-juan,MANG Lai.ANALYZED OF ELA-DRA*EXON2 POLYMORPHISM BETWEEN MONGOLIAN HORSE AND OTHER HOURSE BREEDS[J].Journal of Shandong Agricultural University,2007,38(3):345-350. | |
| Authors: | LI Jin-lian SHI Yon-fei LIU Shu-juan MANG Lai |
| Institution: | 1. Department of biological science and technology, Taishan University Taian 271021 ,China ; 2. College of Animal Science and Veterinary,Inner Mongolia Agricultural University Huhhot 010018 ,China; 3. College of Animal Science and Veterinary Medicine, Shandong Agficultrial University Taian 271018 ,China |
| Abstract: | ELA-DRAexon2 locus was used to detect 296horses from 6 types of three big breeds.The PCR amplified products of the locus were detected by 12 percent non-denatured polyacry lamide gel electrophoresis and showing in silver staining protocol.The results showed that 6 genotypes were detected in 296 horses: 3 homozygotes named AA,BB and CC,respectively and they all had 3 bands;3 heterozygotes named AB,BC and AC,respectively,and they all had 4 bands;AA and BB were dominant genotypes in all horses.The values of He and PIC in Thoroughbred horse and Sanhe horse represent highest polymorphism,4 horse types of Mongolian belonged to intermediate polymorphism.Clustering analysis showed that three big breeds gathered one branch respectively,Xinihe horse and Baerhu horse had nearly relation among Mongolian horse. |
| Keywords: | ELA-DRA*exon2 PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |