Interaction of bovine immunoglobulin gamma 2 (IgG2) with staphylococcal protein A: evidence for IgG2a and IgG2b sub-subclasses in the bovine |
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Authors: | M J Lawman S Joiner D R Gauntlett M D Boyle |
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Institution: | 1. Department of Comparative and Experimental Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610 USA;2. Department of Immunology and Medical Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610, USA;3. Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610, USA |
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Abstract: | The reactivity of bovine IgG with protein A is confusing with respect to which of the bovine IgG class and subclasses are reactive. We have, therefore, re-examined the interaction of bovine immunoglobulins with protein A. The results presented in this paper indicated that at pH 8.0 protein A binds only immunoglobulin of the IgG2 subclass. The bound IgG2 can be readily recovered from an immobilized protein A column at pH 5.0. Furthermore, the antigenic IgG2 eluted demonstrated two charged species which could readily be separated by ion-exchange chromatography. These results indicate that IgG2 in the bovine exists in two sub-subclasses, IgG2a and IgG2b. The two sub-subclasses of IgG2 could be rapidly isolated with a good yield in two-steps namely protein A affinity chromatography followed by ion exchange chromatography. |
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Keywords: | Protein A bovine IgG2 IgG2a IgG2b affinity purification Protéine A bovine IgG2 IgG2a et IgG2b purification par affinité |
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