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| 程序降温法冷冻犬细管精液的实验 | |
| 引用本文： | 禹学礼,栗颖华,昝林森.程序降温法冷冻犬细管精液的实验[J].中国农学通报,2004,20(4):15-15. |
| 作者姓名： | 禹学礼 栗颖华 昝林森 |
| 作者单位： | 1. 河南科技大学动物科技学院,洛阳,471003 2. 洛阳白马寺种公牛站 3. 西北农林科技大学动物科技学院,陕西,咸阳,713029 |
| 基金项目： | 河南省洛阳市重大科技攻关项目(030218). |
| 摘 要： | 分别将6条公犬每次采集的精液（每条采6次），按同样的方法稀释、平衡、分装，而后将分装的细管分为两组，分别采用程序Ⅰ和程序Ⅱ进行冷冻，其冻后活率分别为0.47±0.10和0.34±0.05 (P＜0.01) ；复苏率分别为57.86±8.85和42.27±3.80 (P＜0.01)；顶体完整率分别为34.47±5.20和29.77±4.37(P＜0.01)。结果表明：程序Ⅰ的冷冻效果明显优于程序Ⅱ。 |
| 关 键 词： | 犬 细管精液 冷冻 |
Canine straw semen freezing with programmable freezer |
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| Yu-xueli,Li-yinghua,Zan-linsen.Canine straw semen freezing with programmable freezer[J].Chinese Agricultural Science Bulletin,2004,20(4):15-15. | |
| Authors: | Yu-xueli Li-yinghua Zan-linsen |
| Institution: | （1．College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471003; 2.College of Animal Science and Technology,Northwest Sci-Tech University of Agriculture and Forestry; 3.Luoyang Baimasi herd Bull station,Hena |
| Abstract: | Thirty -six ejaculates from six male dogs were diluted, quilibrated and packed in 0.5ml straws simultaneously, the only difference being in the programs used for freezing. The straws were rozen with programⅠand programⅡin the programmable freezer separately. The mean post thaw progressive motility （PTPM）were 0.47±0.10 and 0.34±0.05 (P＜0.01) resp., and the percentage of sperms with intact acrosome were 34.47±5.20 and 29.77±4.37（P＜0.01)resp. It is concluded that the programⅠwas better than program Ⅱfor freezing canine straw semen. |
| Keywords: | Canine Straw semen Programmable freezing |
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