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平贝母不同表现类型的总生物碱含量测定
引用本文:孙殿奎,刘洋,郭晓雨,田义新,秦佳梅.平贝母不同表现类型的总生物碱含量测定[J].人参研究,2012,24(2):37-38.
作者姓名:孙殿奎  刘洋  郭晓雨  田义新  秦佳梅
作者单位:1. 吉林农业大学中药材学院,吉林长春,130118
2. 通化师范学院生物系,吉林通化,134002
基金项目:吉林省财政厅项目——平贝母品种选育研究和吉林省中医药管理局项目(课题编号:2010-pt072)
摘    要:目的确定平贝母不同表现类型是否在总生物碱含量上存在差别,为其育种做准备。方法超声法提取总生物碱,比色法测定含量。结果平贝母常见的绿茎绿叶品系的总生物碱含量为0.1703%,另2种品系——紫茎紫叶、紫茎绿叶分别为0.1807%、0.1830%。结论不同平贝母表现类型在总生物碱含量上没在显著差异,需要转向对其他性状进行分析。

关 键 词:平贝母  表现型  总生物碱含量  育种

Test on total alkaloid content in the different phenotypes of Fritillaria ussuriensis Maxim
Authors:SUN Dian-kui  LIU Yang  GUO Xiao-yu  TIAN Yi-xinCollege of Traditional Chinese Medicinal Materials  Jilin Agricultural University  ChangChun  Jilin  China QIN Jia-mei
Institution:SUN Dian-kui,LIU Yang,GUO Xiao-yu,TIAN Yi-xin(College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,ChangChun 130118,Jilin,China) QIN Jia-mei(Department of Biology,Tonghua Normal University,Tonghua 134002,Jilin,China)
Abstract:Objective]To identify the differences of the total alkaloid content among the different phenotypes of F.ussuriensis preparing for its breeding.Method]Using ultrasonic extraction for the total alkaloid and colorimetric method for its content.Result]The total alkaloid content in the common phenotype(green stem and leaf) is 0.1703%,the two new others(purple stem and leaf,purple stem and green leaf) are 0.1807%,0.1830%.Conclusion]The different phenotypes of F.ussuriensis show no significant differences in the total alkaloid content,means that the other characteristics need to be researched furtherly.
Keywords:Fritillaria ussuriensis Maxim  Phenotype  Total alkaloid content  Breeding
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