|
|||||
|
|
| 禽流感HA7基因DNA疫苗的构建及免疫保护效果研究 | |
| 引用本文： | 左青山,姜永萍,冉多良,夏俊,李俊辉,王艳丽,陈化兰.禽流感HA7基因DNA疫苗的构建及免疫保护效果研究[J].新疆农业大学学报,2007,30(4):34-37. |
| 作者姓名： | 左青山 姜永萍 冉多良 夏俊 李俊辉 王艳丽 陈化兰 |
| 作者单位： | 1. 新疆农业大学,动物医学学院,乌鲁木齐,830052;中国农业科学院,哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨,150001 2. 中国农业科学院,哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨,150001 3. 新疆农业大学,动物医学学院,乌鲁木齐,830052 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 为了研究禽流感HA7亚型DNA疫苗,将HA7基因插入高效真核表达载体pCAGGS,构建了DNA疫苗质粒pCAGGHA7,然后将pCAGGHA7以100μg剂量一次免疫3周龄SPF鸡,免疫SPF鸡后两周抗体水平达到3Log2,4周后用100 LD50的H7N1鼻腔途径进行攻击毒,发现100μg pCAGGHA7可形成8/8的免疫保护。表明禽流感DNA疫苗pCAGGH7诱导的保护性抗体免疫反应水平较高,能达到免疫保护效果。 |
| 关 键 词： | 禽流感 H5亚型 H7亚型 DNA疫苗 免疫保护 |
| 文章编号： | 1007-8614（2007）04-0034-04 |
| 修稿时间： | 2007-03-06 |
Construction and Protection Efficacy of DNA Vaccine of HA7 Gene of Avian Influenza |
|
| ZUO Qing-shan,JIANG Yong-ping,RAN Duo-liang,XIA Jun,LI Jun-hui,WANG Yan-li,CHEN Hua-lan.Construction and Protection Efficacy of DNA Vaccine of HA7 Gene of Avian Influenza[J].Journal of Xinjiang Agricultural University,2007,30(4):34-37. | |
| Authors: | ZUO Qing-shan JIANG Yong-ping RAN Duo-liang XIA Jun LI Jun-hui WANG Yan-li CHEN Hua-lan |
| Institution: | 1. College of Animal Medicine, Xinjiang Agricultural University, Urumqi, 830052 ; 2. Key Labora- tory of Animal Influenza of the Ministry of Agriculture, Harbin Veterinary Research Institute of CAAS, Harbin, 150001 |
| Abstract: | In this study,the protection efficacy of a plasmid DNA HA7 genes,H7 subtype was cloned into the pCAGGS vector.And this plasmid was used to immunize 3-week-age SPF chickens by 100ug dose and intramuscular injection.The Haemagglutinin inhibition(HI) titer induced by DNA vaccine were tested for 2 weeks.The SPF chickens were challenged with HPAIV H7N1 by the fourth week after the immunization.Four-weeks after the single vaccination,all chickens were challenged with 100 LD50 of HPAIV H7N1;And the result show,100% of protection rate were obtained from the groups immunized with pCAGGHA7 jointly.The study indicated the protection efficacy of a plasmid DNA HA7 genes has perfect. |
| Keywords: | avian influenza H5 subtype H7 subtype DNA vaccine immunization protection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |