| 紫花苜蓿肌动蛋白基因的提取 |
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| 引用本文： | 樊文娜,王成章,严学兵,史鹏飞. 紫花苜蓿肌动蛋白基因的提取[J]. 草原与草坪, 2009, 0(1): 58-60 |
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| 作者姓名： | 樊文娜 王成章 严学兵 史鹏飞 |
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| 作者单位： | 河南农业大学牧医工程学院,河南郑州,450002 |
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| 摘 要： | 选取紫花苜蓿叶片为材料，提取总RNA，利用蒺藜状苜蓿的肌动蛋白基因设计特异性引物，扩增出目的基因的cDNA进行测序，测序结果在NCBI基因库中利用Blast与其他高等植物肌动蛋白基因序列进行了比对、分析，结果证明所提取的基因为紫花苜蓿肌动蛋白基因的片段。
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| 关 键 词： | 紫花苜蓿 肌动蛋白基因 提取 |
Extraction of actin gene from Medicago sativa |
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| FAN Wen-na,WANG Cheng-zhang,YAN Xue-bing,SHI Peng-fei. Extraction of actin gene from Medicago sativa[J]. Grassland and Turf, 2009, 0(1): 58-60 |
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| Authors: | FAN Wen-na WANG Cheng-zhang YAN Xue-bing SHI Peng-fei |
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| Affiliation: | College of Animal Science and Veterinary Medicine;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | The leaf of Medicago sativa was used to extract total RNA.The total RNA was isolated from the leaves of Medicago sativa,and then obtained the objective gene cDNA by using a pair of primers according to actin genes coding region sequence from Medicago truncatula.After the cDNA gene was sequenced,it was blasted in NCBI with other plants.Results indicated that the sequence was the actin gene fragments from Medicago sativa. |
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| Keywords: | Medicago sativa actin gene extraction |
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