In vitro chromosome doubling with colchicine during microspore culture in wheat (Triticum aestivum L.) |
| |
Authors: | NJP Hansen SB Andersen |
| |
Institution: | (1) Department of Agricultural Sciences, Section Plant Breeding and Crop Science, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark |
| |
Abstract: | Isolated microspores of two DH lines of wheat were treated with 8 different colchicine concentrations up to 3 mM for either
24 h or 48 h during microspore culture. Untreated control cultures produced on average 220 embryos per spike (100,000 microspores),
68% of the regenerated plantlets were green, and 15% of the flowering plants were fertile. The colchicine treatments had a
significant effect on chromosome doubling as measured by the percentage of fertile regenerants. Using colchicine concentrations
around 1 mM the percentage of fertile plants among the regenerants was increased up to 53%. The highest number of embryos
and regeneration rates were observed after 24 h colchicine treatment, while the highest frequencies of green plants and fertile
plants were obtained with 48 h colchicine treatments. The highest number of DH plants per spike was found after treatment
with colchicine concentrations of 300 to 1000 μM. Such treatments resulted in an estimated average between the two genotypes
of 23 doubled haploid plants per spike.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | albinism chromosome doubling colchicine treatment green plants haploids microspore embryogenesis T aestivum |
本文献已被 SpringerLink 等数据库收录! |
|