蝉拟青霉杀虫活性成分的分离

 【目的】采用化学分离分析和生物检测相结合的方法，首次从蝉拟青霉Paecilomyces cicadae （Miq.）Samson]子实体提取物中分离出对小菜蛾Plitella xylostella （Lepidoptera：Plutellidae）]具有杀虫活性的化合 物。【方法】在活性检测引导下，蝉拟青霉子实体40%乙醇提取物经二氯甲烷、乙酸乙酯、正丁醇溶剂依次萃取（剩余溶液为水溶组分），再经过大孔树脂、HPLC和Sephedex LH-20分离纯化得到杀虫活性化合物。【结果】分离纯化到一 个白色粉末状化合物，浓度10 mg•ml-1，24、48和72 h时校正死亡率分别为（95.1±1.8）%、（97.7±1.6）%和（99.1±1.7）%。【结论】该化合物具有较好的杀小菜蛾活性，对酸稳定，对胃蛋白酶不敏感，并具有茚三酮不显色等其它理化性质。

Separation of Insecticidal Ingredient of Paecilomyces cicadae (Miquel) Samson

Insecticidal active substance of the fruiting body of Paecilomyces Cicadae（Miquel）Samson was isolated and purified by combined with separation and analyzing and biology experiment for the first time. The results showed that the water portion of ethanol-H20 total extract was strong toxicity to against the third instar larvae of Plutella xylostella L. in 24h, 48h and 72h with the corrective mortality of 46.67±1.34%、60±2.24% and 66.67±1.79%, respectively, at the concentration of 50.0mg/ml, and the other fractions of CH2Cl2, ethyl acetate and n-butanol were weak. By means of bioactive guided isolation of macroporous adsorption resin column, high performance liquid chromatography and sephadex A-20 column, a white powder compound was obtained. At the concentration of 10.0mg/ml, the corrective mortality of the compound against Plutella xylostella L were significantly ( P < 0101), 95.12±1.83%, 97.67±1.60% and 99.06±1.68%, respectively, in 24h, 48h and 72h. The bioactive compound was stable to acids and was not sensitive to proteinase pepsin, other physical and chemical chractristics were reported.