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耐盐好氧反硝化细菌X12的分离及反硝化特性
引用本文:司圆圆,陈兴汉,许开航,余祥勇.耐盐好氧反硝化细菌X12的分离及反硝化特性[J].农业工程,2017,7(2):68-71.
作者姓名:司圆圆  陈兴汉  许开航  余祥勇
作者单位:1.阳江职业技术学院,阳江529500;广东海洋大学,湛江524025
基金项目:广东省科技计划项目广东省海洋经济动物良种繁育与健康养殖院士工作站(2013B090400017),广东省扬帆计划重点人才工程(C0035000),2013年扬帆计划引进创新团队项目(201312H10)
摘    要:从阳江东平某虾池过滤口筛选出一种脱氮性能较高的好氧反硝化细菌X12,为革兰氏阴性菌、杆菌。对X12进行了不同条件下反硝化的特性研究。结果表明,碳源为柠檬酸钠、温度30 ℃、转速160 r/min,pH=7.0,以亚硝酸钠为唯一氮源时,反硝化脱氮效率为96.5%。后续通过逐步提高培养基中氮素的浓度,以及对该菌株进行驯化及固定化技术,以期在海水养殖废水的脱氮处理方面具有更大潜力。 

关 键 词:好氧反硝化    生物脱氮    去除率    养殖废水

Isolation and Denitrification Characteristics of Salt-tolerant Aerobic Denitrifying Bacterial Strain X12
Si Yuanyuan,Chen Xinghan,Xu kaihang and Yu Xiangyong.Isolation and Denitrification Characteristics of Salt-tolerant Aerobic Denitrifying Bacterial Strain X12[J].Agricultural Engineering,2017,7(2):68-71.
Authors:Si Yuanyuan  Chen Xinghan  Xu kaihang and Yu Xiangyong
Abstract:A heterotrophic nitrification-aerobic denitrification bacterium named X12 was isolated from the filter mouth of Dongping shrimp pond,Gram negative bacteria,bacillus.The characteristics of denitrification of X12 was studied under different conditions and the optimal conditions for its growth and denitrification were followed as carbon source was sodium citrate,30 ℃,160 r/min,pH 7.0.Increasing of nitrogen concentration in the medium and immobilization technology were to be studied in the subsequent step.This strain showed great potential in biological nitrogen removal of wastewater with high salt from mariculture and industries. 
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