普拉索芦荟组织培养技术研究

取普拉索芦菩嫩茎作外植体，培养于附加不同种类和激素浓度的ＭＳ或改良的ＭＳ培养基上，在附加 ６－ＢＡ１．５ ｍｇ／Ｌ时，丛生芽诱导效果最佳，萌发数量最多；在附加 ６－ＢＡ１．０ｍｇ／Ｌ＋ＮＡＡ０．３ｍｇ／Ｌ时，丛生芽生长最好，芽长且健壮，；在附加Ｉ ＡＡ０．２ ｍｇ／Ｌ时，生根效果最佳；当苗高 ３ ｃｍ以上，根长达２ ｃｍ以上时，开盖 ３ ｄ移入温室（１０～１６℃），炼苗 ３ ｄ后，移栽于蛙石；河沙；腐质土为０．５：１：１．５的混合基质的土壤中，成活率达９１．３％。

Study on the Technology of Tissue Culture in Aloe Vera Var Chinensis

Take young stems of Aloe vera var chinensis as explant ,breed additional various kinds and hormone MS of concentration or based on improved MS with culture medium while adding 6- BA 1. 5mg/L, the effect of inducement of overgrown bud is optimum and sprout quantity is the most; growth of overgrown bud is the best and bud is length and also healthy while adding 6- BA 1. 0mg/L + NAA 0.3 mg/L; when seedlings height are above 3 cm and the root length reaches over 3 cm, the effect of root develops well while adding NAA 0.2 mg/L; open lid for 3 days and then move it into greenhouse (10- 16 °C), and train seedlings after 3 days and then transplant them into vermiculite; sandy bed; humus soil with mixture medium soil by 0. 5:1:1.5, and survival rate reaches 91.3%.