樟子松松针石蜡切片制备

[目的]以樟子松松针为材料，探索一套适合樟子松松针制备石蜡切片的条件。[方法]在传统植物石蜡切片技术的基础上，结合松针的结构特点，对固定、脱水、粘片、染色等环节进行了系列摸索。[结果]FAA固定剂配比中采用70%乙醇；粘片时材料置于40℃水浴锅中展开，用防脱载玻片捞取，置35℃烘箱24 h；松针切8μm厚度,染色条件为伊红5 min，苏木精1 min。[结论]获得的条件可强化固定和脱水效果，粘片操作简便，不易脱片，染色效果较好。

Preparation of Paraffin Section of Pinus sylvestris Needles

[Objective] Taking Pinus sylvestris needles as materials, a set of test con-ditions suitable for the preparation of paraffin sections of Pinus sylvestris needles was determined. [Method] Based on the traditional method for paraffin sections preparation, steps including fixation, dehydration, adhering to slides and staining were investigated taking the structural characteristics of pine needles into considera-tion. [Result] 70% ethanol was used in the FAA fixative; before affixed on slides, the cut sections were first expanded in a 40 ℃ water bath and taken out by using Su-perfrost Plus slides, fol owed by drying in a 35 ℃ oven for 24 h; pine needles were cut into 8 μm thick sections; staining was achieved by immersion in eosin for 5 min and in hematoxylin for 1 min. [Conclusion] The conditions obtained above can en-hance the effect of fixation and dehydration; adhering to slides is easy to operate and the sections are not easy to drop; the dyeing effect is relatively preferable.