| 影响小麦K199不同外植体愈伤组织诱导的因素 |
| |
| 引用本文： | 张弛,李霞,王玉珍.影响小麦K199不同外植体愈伤组织诱导的因素[J].华北农学报,2008,23(Z1). |
| |
| 作者姓名： | 张弛 李霞 王玉珍 |
| |
| 作者单位： | 1. 中国科学院,遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021;中国科学院研究生院,北京,100039
2. 中国科学院,遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021 |
| |
| 基金项目： | 中国科学院知识创新重大项目 |
| |
| 摘 要： | 以小麦科农199(K199)的完整种子、离体成熟胚和幼叶作为外植体,探讨了取材时间、取材部位、激素浓度和培养方式对小麦离体培养的影响。结果表明,刨碎的离体成熟胚和叶片基段为小麦组织培养的良好起始材料,种子萌发4 h为最佳取材时间,2 mg/L 2,4-D更有利于获得II型愈伤,光培养比暗培养更适用于叶片愈伤培养。
|
| 关 键 词： | 小麦 组织培养 成熟胚 幼叶 |
Factors Affect Callus Induction from Various Explants of K199 |
| |
| ZHANG Chi,LI Xia,WANG Yu-zhen.Factors Affect Callus Induction from Various Explants of K199[J].Acta Agriculturae Boreali-Sinica,2008,23(Z1). |
| |
| Authors: | ZHANG Chi LI Xia WANG Yu-zhen |
| |
| Institution: | ZHANG Chi1,2,LI Xia1,WANG Yu-zhen1 |
| |
| Abstract: | The explants used in the study are whole seed,detached mature embryo and leaf segment of wheat cultivar K199.The factors affecting the induction and differentiation of calli were studied.The results showed that mature embryo pieces and basal segments of young leaves were the best explant sources for K199.Good callus with high regeneration frequency could be obtained from medium containing Kinetin(KT)3 mg/L and 2,4-D ichlorophenoxyacetic acid(2,4-D) 0.01 mg/L. |
| |
| Keywords: | Wheat(Triticum aestivum L ) Tissue culture Mature embryo Young leaf |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
