Light and Scanning Electron Microscopy Studies on the Infection of Oriental Lily Leaves By Botrytis Elliptica |
| |
Authors: | Ting Fang Hsieh Jenn Wen Huang Tom Hsiang |
| |
Institution: | (1) Department of Plant Pathology, Taiwan Agricultural Research Institute, Wu-feng, 413 Taichung, Taiwan;(2) Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan;(3) Department of Environmental Biology, University of Guelph, Ontario, Canada |
| |
Abstract: | Light, scanning electron and fluorescent microscopy were used to observe the infection process of Botrytis elliptica on leaves of oriental lily (cv. Star Gazer). At 20 °C and 100% relative humidity, conidia germinated on both adaxial and abaxial foliar surfaces, but germ tubes failed to invade epidermal cells on the adaxial surface. On abaxial surfaces, short (< 20 m) swollen germ tube appressoria penetrated through stomatal openings (19%), through the epidermis near guard cells (52%), or directly through epidermal cells (29%). Esterase activity was detected on germ tubes and conidia after 6 h of incubation, and deformation of the cuticle on abaxial surfaces of lily was observed surrounding infection sites. By 3 h after inoculation, almost 70% of the conidia had germinated, but no penetration was observed. At 6 h after inoculation, almost one-third of germinated conidia had penetrated epidermal cells, and water-soaked lesions were associated with 20% of the penetrations. By 9 h after inoculation, approximately 60% of the germinated conidia had penetrated plant tissues, and water-soaked lesions were associated with 60% of the infections. Fluorescent microscopy with a specific fungal stain allowed assessment of successful infection and visualization of sub-epidermal hyphae. We conclude that penetration of abaxial foliar surfaces of oriental lilies by B. elliptica occurs via short swollen germ tube appressoria mostly near stomata. |
| |
Keywords: | direct penetration fluorescent stain infection process swollen germ tube appressoria lobed or digitate appressoria |
本文献已被 SpringerLink 等数据库收录! |
|