| 新发林麝环状类病毒的鉴定和分析 |
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| 引用本文： | 刘齐,杨世兴,张文,齐敦武,付为国. 新发林麝环状类病毒的鉴定和分析[J]. 中国动物传染病学报, 2022, 0(1): 39-43 |
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| 作者姓名： | 刘齐 杨世兴 张文 齐敦武 付为国 |
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| 作者单位： | 江苏大学农业工程研究院;江苏大学医学院;成都大熊猫繁育研究基地四川省濒危野生动物保护生物学重点实验室 |
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| 基金项目： | 国家重点研发计划(2017YFC1200201);国家自然基金(81741062)。 |
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| 摘 要： | 利用病毒宏基因组学(viral metagenomics)方法,本研究从95份林麝粪便样品中检测到1株新型圆环病毒样(Circovirus-like)病毒,经过拼接获得其全基因组序列,该毒株命名为ls-cyc.其基因组全长约3552 nt,GC含量39.00％,含有2个开放阅读框(ORF),即ORF1和ORF2.其中O...
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| 关 键 词： | 林麝 环状病毒 病毒宏基因组学 基因组结构分析 系统发育分析 |
Discovery of a Novel Circovirus-like Virus in Feces of Forest Musk Deer Using Viral Metagenomics |
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| LIU Qi,YANG Shixing,ZHANG Wen,QI Dunwu,FU Weiguo. Discovery of a Novel Circovirus-like Virus in Feces of Forest Musk Deer Using Viral Metagenomics[J]. Chinese Journal of Animal Infectious Diseases, 2022, 0(1): 39-43 |
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| Authors: | LIU Qi YANG Shixing ZHANG Wen QI Dunwu FU Weiguo |
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| Affiliation: | (Agricultural Engineering Research Institute,Zhenjiang 212013,China;School of Medicine,Jiangsu Zhenjiang 212013,China;Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife,Research Base of Giant Panda Breeding,Chengdu 610081,China) |
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| Abstract: | A novel circovirus-like virus was discovered from 95 fecal samples of musk deer using viral metagenomics method.The newly discovered virus was designated as ls-cyc.The complete genome sequence was acquired by sequence assembly,of which the genome length was 3552 nt and the GC content was 39.00%.Genomic structure analysis showed that the virus contained two open reading frames,namely ORF1 and ORF2.The ORF1 length was 1386 nt and ORF2 was 1953 nt.The amino acid sequence encoded by ORF1 showed 34.31%similarity to a pig serum circular circovirus(MH170058.1).Phylogenetic analysis based on the REP protein showed that ls-cyc shared the same evolutionary branch as the circular circoviruses from pigs(MH170058.1)and ducks(NC_030134.1).Further epidemiological studies were needed to determine whether the virus was associated with specifi c diseases of musk deer. |
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| Keywords: | Forest musk deer viral metagenomics circovirus genomic structure phylogenetic analysis |
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