Interaction between the helicase domain of the tobacco mosaic virus replicase and a tobacco arginine decarboxylase |
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| Authors: | Takumi Shimizu Yasuyuki Yamaji Yoshitake Ogasawara Koji Hamada Keitaro Sakurai Toshihiko Kobayashi Takato Watanabe Tadaaki Hibi |
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| Institution: | (1) Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) Present address: National Agricultural Research Center, Ibaraki, Japan |
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| Abstract: | In a yeast two-hybrid screening test for tobacco proteins that interact with TMV replicase using the helicase (H) domain as bait, a cDNA clone was selected that encodes a polyamine biosynthetic enzyme, arginine decarboxylase (ADC). In yeast cells, the C-terminal internal region of ADC interacted with the H domain. This observation was confirmed in vitro by far-Western blotting. Inhibition of the binding between the H domain and the IRnHEL (I region and N-terminus of helicase domain) region by ADC using a yeast three-hybrid assay suggested possible interference of the heterodimerization of 126 K and 183K by ADC.The nucleotide sequence data of pADCF reported in this study is available in the DDBJ/EMBL/GenBank databases under accession number AB110952 |
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| Keywords: | Tobacco mosaic virus Replicase Yeast two-hybrid screening Arginine decarboxylase Nicotiana tabacum |
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