| 一株约翰逊不动杆菌的分离和鉴定 |
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| 引用本文： | 王恒安,秦磊,成志恒,赵国屏.一株约翰逊不动杆菌的分离和鉴定[J].南京农业大学学报,2004,27(2):139-142. |
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| 作者姓名： | 王恒安 秦磊 成志恒 赵国屏 |
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| 作者单位： | 1. 上海交通大学农业与生物学院,上海,201101;中国科学院上海生物工程研究中心,上海,200233
2. 中国科学院上海生物工程研究中心,上海,200233 |
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| 基金项目： | 国家自然科学基金项目 ( 3 0 0 70 0 16)，国家 863攻关课题 ( 2 0 0 1AA2 3 40 5 1) |
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| 摘 要： | 在土壤中分离到一株细菌STRB，该菌可以在SFM、LB、EMB、PDA、MM、MacC等多种培养基上生长，革兰氏染色为阴性，电镜观察无鞭毛。生化指标检测显示，该菌株对多数糖类不能发酵，经bioMerieux Vitek公司的革兰氏阴性细菌检测试验卡(GNI)鉴定为醋酸钙不动杆菌。设计2对简并引物对其16S rRNA进行PCR扩增，16S rRNA序列及其进化树分析结果显示，该菌为约翰逊不动杆菌。
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| 关 键 词： | 约翰逊不动杆菌 鉴定 16S rRNA |
| 文章编号： | 1000-2030(2004)02-0139-04 |
| 修稿时间： | 2003年8月20日 |
Identification of an isolate of Acinetobacter johnsonii |
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| WANG Heng-an ,QIN Lei ,CHENG Zhi-heng ,ZHAO Guo-ping.Identification of an isolate of Acinetobacter johnsonii[J].Journal of Nanjing Agricultural University,2004,27(2):139-142. |
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| Authors: | WANG Heng-an QIN Lei CHENG Zhi-heng ZHAO Guo-ping |
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| Institution: | WANG Heng-an 1,2,QIN Lei 2,CHENG Zhi-heng 2,ZHAO Guo-ping 2* |
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| Abstract: | A bacterial strain named STR B was isolated from soil,which could be cultivated on some media,such as SFM,LB,EMB,PDA,MM and MacC agar.It was gram negative and could be viewed without flagellas under electron microscope.The strain could not ferment most of sugars in the biochemical test and it was identified as Acinetobacter calcoaceticus with the gram negative identification card(GNI)of bioMerieux Vitek Company.Two pairs of primers were designed for PCR of its 16S rRNA.The sequence analysis results of 16S rRNA sequence with online software showed that the STR B belonged to Acinetobacter johnsonii,not a strain of Acinetobacter calcoaceticus. |
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| Keywords: | Acinetobacter johnsonii identification 16S rRNA |
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