| 莆田黑猪猪耳皮肤成纤维细胞系的建立 |
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| 引用本文： | 张文昌,王秀爱,刘凤军,庄益芬,马群,邹长连,洪志勇,陈梅芳,陈曦,谢旭东,叶屹峰,余翠月. 莆田黑猪猪耳皮肤成纤维细胞系的建立[J]. 福建畜牧兽医, 2012, 0(4): 9-11 |
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| 作者姓名： | 张文昌 王秀爱 刘凤军 庄益芬 马群 邹长连 洪志勇 陈梅芳 陈曦 谢旭东 叶屹峰 余翠月 |
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| 作者单位： | 1. 福建农林大学动物科学学院 福州 350002
2. 河南科技大学动物科技学院 洛阳 471003 |
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| 基金项目： | 福建省科技计划项目（2008N0001）资助 |
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| 摘 要： | 为获得可用于体细胞核移植中需要的供核细胞,利用组织块法培养莆田黑猪猪耳皮肤成纤维细胞,并对莆田黑猪猪耳皮肤成纤维细胞进行传代及冷冻.通过试验,获得能在体外正常传代培养的细胞系,为莆田黑猪克隆研究提供供核细胞来源.
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| 关 键 词： | 莆田黑猪 成纤维细胞 体外培养 冷冻 |
Establishment of Putian Black Piglets Ear Skin Fibroblast Cell Line |
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| Zhang Wenchang Wang Xiuai Liu Fengjun Zhuang Yifen Ma qun Zou Changlian Hong Zhiyong Chen Meifang Chen Xi Xie Xudong Ye Yifeng Yu Cuiyue. Establishment of Putian Black Piglets Ear Skin Fibroblast Cell Line[J]. Fujian Journal of Animal Husbandry and Veterinary medicine, 2012, 0(4): 9-11 |
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| Authors: | Zhang Wenchang Wang Xiuai Liu Fengjun Zhuang Yifen Ma qun Zou Changlian Hong Zhiyong Chen Meifang Chen Xi Xie Xudong Ye Yifeng Yu Cuiyue |
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| Affiliation: | Zhang Wenchang Wang Xiuai Liu Fengjun Zhuang Yifen Ma qun Zou Changlian Hong Zhiyong Chen Meifang Chen Xi Xie Xudong Ye Yifeng Yu Cuiyue (1.College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002; 2.College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003) |
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| Abstract: | In order to get donor cells for somatic cells nuclear transfer , Tissue piece culture dissociate ear skin fibroblast in Putian black pigs in this study, and transfer of culture and freezing.Through the study, We gained cell lines that can normally serial subeuhivation in vitro which offer cell resources for Putian black pig clone research. |
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| Keywords: | Putian black pig Fibroblast in vitro culture Freezing |
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