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| 油菜BnERF1基因的克隆及其过表达载体的构建 | |
| 引用本文： | 潘宣丞,杜雪竹. 油菜BnERF1基因的克隆及其过表达载体的构建[J]. 湖北农业科学, 2017, 56(10). DOI: 10.14088/j.cnki.issn0439-8114.2017.10.042 |
| 作者姓名： | 潘宣丞 杜雪竹 |
| 作者单位： | 湖北大学生命科学学院,武汉,430062 |
| 摘 要： | 利用拟南芥ERF1基因的序列,在NCBI数据库中检索油菜(Brassica napus)ERF1基因的序列信息,并设计特异性引物,PCR扩增油菜BnERF1基因。结果表明,成功扩增出油菜BnERF1基因,并构建了过表达载体pCAMBIA2301-ERF1,为进一步研究BnERF1的抗病分子机理和选育新的抗病油菜种质奠定了试验基础。 |
| 关 键 词： | 油菜(Brassica napus) ERF1 克隆 载体 |
Cloning of BnERF1 Gene from Rape and Construction of Its Overexpression Vector |
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| PAN Xuan-cheng,DU Xue-zhu. Cloning of BnERF1 Gene from Rape and Construction of Its Overexpression Vector[J]. Hubei Agricultural Sciences, 2017, 56(10). DOI: 10.14088/j.cnki.issn0439-8114.2017.10.042 | |
| Authors: | PAN Xuan-cheng DU Xue-zhu |
| Abstract: | By the sequence of Arabidopsis thaliana ERF1, the sequence information of Brassica napus ERF1(BnERF1) was re-trieved in NCBI database. According to the sequence that we found, the unique primer for amplification BnERF1 was de-signed. Results were confirmed that the gene of BnERF1 and the overexpression vector pCAMBIA2301-ERF1 were successful-ly obtained. It laid foundation for further research on disease resistance molecular mechanism of BnERF1 and breeding new disease-resistant rape germplasm. |
| Keywords: | Brassica napus ERF1 cloning vector |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |