人工合成甘蓝型油菜高频率再生研究初报

资源材料保存在作物遗传资源的研究与利用中起到非常重要的作用。该实验以不同来源的人工合成甘蓝型油菜KH106,KH109,KH111D为材料,以花序轴,子房,果柄为外植体研究其再生情况,结果表明,由芥蓝和小白菜人工合成的甘蓝型油菜KH106的再生频率极显著高于由羽衣甘蓝和白菜型油菜人工合成的甘蓝型油菜KH109和KH111;与花序轴和子房为外植体再生频率相比,以果柄为外植体平均再生频率最高,差异达到极显著水平;利用不同预处理培养基处理不同时间,结果显示不经过预处理直接接种到分化培养基上再生频率最高。

The Primal Study on Enhancing Regeneration Frequency of Resynthesized Brassica napus

The preservation of crops materials plays very important role in research and utilization of resources. In this paper, artificial synthesis Brassica napus, named KH106, KH109 and KH111D, has been separately studied regeneration capability of scape, germen and flower stalk as explants. It showed that KH106, which derives from B. albograbra Bailey × B. Parichinensis, had higher regeneration frequency than other two lines KH109 and KH111D, which derive from B.oleracea Var aceaphala × B.campetris. As for different explants, average regeneration frequency of flower stalk was higher with significant level than others. In addition, Some different explants were pretreated with different medium for different periods, the result indicated that regeneration frequency is higher when explants were not pretreated and transited directly on the differentiation medium.