活性炭对半乳甘露低聚糖的脱色研究

旨在通过活性炭对半乳甘露低聚糖溶液进行脱色精制,从而提高半乳甘露低聚糖的纯度.首先,运用单因素试验和正交试验方法,获得最佳的脱色条件,最后对脱色后的产物进行红外分析及吸附等温线的测定.研究发现:相比于粉末活性炭和柱状活性炭,颗粒活性炭的选择性脱色精制效果最佳,因此选择颗粒活性炭进行下一步的单因素试验和正交优化试验.首先...

Investigation of decolorization of galactomanno-oligosaccharides by activated carbon

In recent years,galactomanno-oligosaccharides(GMOS)have received more and more attention due to their excellent immune-enhancing effects and the function of regulating intestinal flora.Therefore,more researchers are devoted to the preparation of GMOS,however the obtained crude GMOS usually contain impurities such as proteins and pigments.In order to expand the application of GMOS to the fields of food and medicine,the decolorization and purification of GMOS is necessary.Herein,it is expected to decolorize and refine the GMOS solution by activated carbon,thereby improving the purity of the GMOS.Firstly,the single factor test,including activated carbon type,addition amount,adsorption temperature and adsorption time,was carried out,followed by the orthogonal test,to obtain the best decolorization conditions.Moreover,The infrared analysis and adsorption isotherm determination analysis were used to characterize the decolorized product.In the selection of activated carbon type,compared with the powdered activated carbon and columnar activated carbon,the granular activated carbon had the best selective decolorization effect.Therefore,the granular activated carbon was selected for the next single factor and orthogonal optimization experiment.Firstly,the conditions were preliminarily screened through single-factor experiments,and then the selected conditions were orthogonally optimized.Consequently,the best conditions of the addition amount,adsorption temperature and adsorption time were 8%,45℃,and 70 min,respectively.At this time,the removal rates of phenolic pigments and Maillard pigments were 91.08%and 66.52%,respectively,followed by the recovery rate of GMOS of 93.53%.Then,through the infrared analysis,it was found that the activated carbon did not change the structure of polysaccharide sugar residues while decolorizing.In addition,the analysis of adsorption isotherms indicated that the adsorption of granular activated carbon for pigments belongs to type I isotherm,namely Langmuir isotherm.More importantly,the saturated adsorption capacities of granular activated carbon for phenolic pigments and Maillard pigments were 0.32 and 0.57 g/g,respectively.Overall,the experimental results show that the granular activated carbon can be used to remove the pigment in the GMOS solution,as well as minimizing the loss of GMOS.This research lays the foundation for the application of GMOS in the food field.