| 高产淀粉酶芽孢杆菌菌株的筛选 |
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| 引用本文： | 唐丽江,王振华,王迪. 高产淀粉酶芽孢杆菌菌株的筛选[J]. 安徽农业科学, 2009, 37(12): 5362-5363 |
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| 作者姓名： | 唐丽江 王振华 王迪 |
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| 作者单位： | 成都农业科技职业学院畜牧兽医分院,四川温江,611130;成都农业科技职业学院畜牧兽医分院,四川温江,611130;成都农业科技职业学院畜牧兽医分院,四川温江,611130 |
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| 摘 要： | [目的]筛选高产淀粉酶的芽孢杆菌菌株。[方法]选用10株芽孢杆菌，采用染色法初选和3，5-二硝基水杨酸显色法复选对其产淀粉酶的能力进行测定，筛选高产淀粉酶的芽孢杆菌菌株。[结果]10株芽孢杆菌菌株中有9株具有产淀粉酶能力，其中编号为Pab03、Pab02的2株枯草芽孢杆菌产酶活性最高，染色圈直径／菌落直径分别为2．284、1．961；在未作发酵条件优化的前提下，其酶活力分别达到（31．331±0．985）、（21．521±1．390）U，在作为新型的消化酶饲料添加剂方面具有广阔的应用前景。而菌株凝结芽孢杆菌PSAF1和枯草芽孢杆菌PJK01所产淀粉酶活力最低，分别为（0．366±0．022）、（0．704±0．089）U。[结论]成功筛选了2株高产淀粉酶的枯草芽孢杆菌菌株，分别为Pab03和Pab02。
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| 关 键 词： | 淀粉酶 芽孢杆菌 酶活力 筛选 |
Research on the Screening of Bacillus Strain Producing High-yielding Amylase |
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| TANG Li-jiang et al. Research on the Screening of Bacillus Strain Producing High-yielding Amylase[J]. Journal of Anhui Agricultural Sciences, 2009, 37(12): 5362-5363 |
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| Authors: | TANG Li-jiang et al |
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| Affiliation: | TANG Li-jiang et al(Branch of Animal Husb,ry , Veterinary Medicine,Chengdu Vocational College of Agricultural Science , Technology,Wenjiang,Sichuan 611130) |
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| Abstract: | [Objective] The research on the screening of Bacillus strain with the ability in high-yielding amylase was conducted.[Method] The capacity of 10 Bacillus strains producing amylase was primarily tested with the staining method and finally determined with the chromogenic method of 3,5-dinitro-salicylic acid for the screening of the Bacillus strain with the ability in high-yielding amylase. [Results] 9 Bacillus strains had the capacity of amylase production,among which,two Bacillus subtilis strains: Pab03 and ... |
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| Keywords: | Amylase Bacillus Enzyme activity Screening |
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