来自簇毛麦抗条锈病新基因的SSR标记

 用小麦条锈菌条中30号生理小种,对小麦抗病种质小麦-簇毛麦易位系V9128-1和铭贤169的杂交后代进行抗条锈性遗传分析,小麦-簇毛麦易位系V9128-1的抗病性符合1对显性抗条锈病基因控制。并根据F₂抗、感病单株分离比例组建抗感池,用SSR技术寻找与抗病基因连锁的分子标记。从121个SSR引物组合中筛选到2个与抗病基因YrV1(暂命名)紧密连锁的微卫星标记Xgwm566和Xgwm376,遗传距离分别为3.6和5.5cM;因此,该抗条锈病基因位于小麦3B染色体短臂上。这2个标记不仅能在小麦-簇毛麦易位系V9128-1中检测到,而且在抗病基因供体亲本簇毛麦中也能检测到。综合抗病基因来源和分子生物学试验结果,可以推断,YrV1很可能是1个来自簇毛麦并与已知抗条锈病基因不同的新基因。

Microsatellite tagging of stripe-rust resistance gene YrV1 derived from Haynaldia villosa

The progeny population,developed by crossing V9128-1 translocation lines(Triticum aestivum-Haynaldia villosa) with susceptible cultivar MingXian169,was investigated with yellow rust race CY30.A single dominant gene was confirmed by a 3:1 segregation ratio for F₂ population.The parents and the resistant and susceptible bulks were used for screening 121 SSR primer combinations.The markers,Xgwm566 and Xgwm376,were tightly linked to YrV1(temporarily designated).YrV1 was 3.6 cM from Xgwm566,and 5.5 cM from Xgwm376.Therefore,YrV1 was located in the short arm of chromosome 3B.Two markers were tested both the genomic DNA of V9128-1 and H.villosa(one of the original parents of V9128-1).By pedigree and molecular marker analysis of YrV1,the results indicated that YrV1 derived from H.villosa was likely a novel Yr gene.