SSR技术及其在果树上的应用

SSR(Simple sequence repeat)技术以其丰富的多态性、共显性遗传、重复性好和操作简便等优点日益受到重视,已成为植物遗传和育种研究中不可缺少的分子标记。对SSR技术的原理和特点作了简要的介绍,较详细地分析了如何获得SSR引物,特别是综述了果树上SSR引物的研究现状,同时将其与其它几种主要的分子标记进行了比较分析,认为SSR标记检测的位点多态性水平明显高于RFLP,而且重复性优于RAPD;着重介绍了SSR技术在果树种质资源和构建果树遗传图谱及基因定位等研究中的应用现状;指出SSR技术将在果树科研上起到重要的作用。

SSR Technique and Development of the Study in Fruit Science

Simple sequence repeat (SSR) markers are highly polymorphic, codominant, well-repeated and convenient. It has become a useful molecular marker technique in studying plant genetics and breeding.The theory and characteristics of SSR and the differences between SSR marker and the other main molecular markers are introduced; besides these, how to obtain the SSR primers are discussed as well. The polymorphism of loci identified by SSR technique is higher than RFLP, and the repeatability is better than RAPD.This paper is emphasized on the application of SSR marker in fruit germplasm, genetic linkage map and gene locating studying. SSR technique will play an important role in fruit science research.