饲料添加苜蓿草粉对刺参幼参生长、体成分及免疫酶的影响

为了探究紫花苜蓿草粉的适宜添加比例,研究了苜蓿草粉的添加水平依次为0(对照),5%(A1),10%(A2),15%(A3)和20%(A4)的5种饲料对刺参幼参(3.16±0.66)g生长、体成分和免疫酶活性的影响。结果显示,刺参特定生长率(SGR)的最大值(0.73%)和饵料系数(FCR)的最小值都出现在A2组。A2组刺参的粗蛋白含量最高(52.10%),显著高于A1与A4组(P0.05)。A4组刺参的过氧化氢酶(CAT)活力值最高(142.28U/mL),显著高于A0、A1与A2组(P0.05);A3组刺参的超氧化物歧化酶(SOD)活力值最高(136.58U/mL),显著高于A0组(P0.05);A3组刺参的溶菌酶(LZM)含量最高(1.92μg/mL),显著高于A0、A1与A2组(P0.05)。结果表明,当苜蓿草粉的添加比例为10%左右时,实验刺参的养殖效果最佳。

Effects of alfalfa meal on growth,body composition and immune enzymes in Apostichopus japonicus

To determine the appropriate proportion of Medicago sativa in the diet of Japanese sea cucumber (Apostichopus japonicus), the effects of five diets containing zero (A₀), 5% (A₁), 10% (A₂), 15% (A₃) and 20% (A₄) M. sativa meal on growth, body composition and immune enzyme activity in A. japonicus (3.16±0.66 g) were assessed. The A₂ diet produced the highest growth rate (0.73%), feed conversion ratio (FCR) and crude protein content (52.10%), significantly higher (P<0.05) than the A₁ and A₄ diets. The activity of catalase in sea cucumbers from group A₄ (142.28 U/mL) was significantly higher than those in groups A₀, A₁ and A₂ (P<0.05). Superoxide dismutase (SOD) activity in A₃ (136.58 U/mL) was significantly higher than the A₀ group (P<0.05). Additionally, lysozyme (LZM) content in the A₃ group (1.92 μg/mL) was significantly higher than those in groups A₀, A₁ and A₂ (P<0.05). The results suggest that the optimum proportion of M. sativa in A. japonicus diets is 10%.