Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos |
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Authors: | Kozo Inaba Masaharu Seno Hisaya Morino |
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Institution: | (1) Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14 Hyogo, Japan;(2) Department of Bioengineering Science, Faculty of Engineering, Okayama University, 700 Tsushima, Okayama, Japan |
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Abstract: | Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4–8 and 12–16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12–16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4–8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4–8 somite embryos. In the heparin-binding fraction derived from 12–16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable. |
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Keywords: | fish FGF-1 fish FGF-2 fish embryos cell proliferation heparin affinity Western blot |
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