| 极细链格孢菌蛋白激发子PeaT1在酵母双杂交系统中转录激活活性的检测（英文） |
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| 作者单位： | LIU Yan-feng,QIU De-wen,ZENG Hong-mei,YANG Xiu-fen Institute of Plant Protection,CAAS,Beijing 100081 |
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| 摘 要： | The peaT1 gene fragment was amplified from pGEM-6p-1-peaT1 by PCR,and recovered target gene was cloned into pLexA vector.After digestion and sequencing,the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC,and the transcriptional activity of bait vector was detected.The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed,for the two bands of recombinant bait plasmid in agarose gel electrophoresis were expected after digesting by restriction endonuclease EcoR I and Xho I.Therefore,the recombinant bait plasmid could be used in yeast two-hybrid system to screen a cDNA library.
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Detection for Transcriptional Activity of Alternaria Tenuissim Protein Elicitor in Yeast Two-hybrid System |
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| LIU Yan-feng,QIU De-wen,ZENG Hong-mei,YANG Xiu-fen Institute of Plant Protection,CAAS,Beijing. Detection for Transcriptional Activity of Alternaria Tenuissim Protein Elicitor in Yeast Two-hybrid System[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2008, 0(1) |
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| Authors: | LIU Yan-feng QIU De-wen ZENG Hong-mei YANG Xiu-fen Institute of Plant Protection CAAS Beijing |
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| Affiliation: | LIU Yan-feng,QIU De-wen,ZENG Hong-mei,YANG Xiu-fen Institute of Plant Protection,CAAS,Beijing 100081 |
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| Abstract: | The peaT1 gene fragment was amplified from pGEM-6p-1-peaT1 by PCR,and recovered target gene was cloned into pLexA vector.After digestion and sequencing,the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC,and the transcriptional activity of bait vector was detected.The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed,for the two bands of recombinant bait plasmid in agarose gel electrophoresis were expected after digesting by restriction endonuclease EcoR I and Xho I.Therefore,the recombinant bait plasmid could be used in yeast two-hybrid system to screen a cDNA library. |
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| Keywords: | PeaT1 Yeast two-hybrid Transcriptional activity |
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