反义抑制ghrelin对VEGF及其受体Fit-1 mRNA表达的影响

［目的］ 探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1 mRNA表达的影响。［方法］ 试验分为4组：Ⅰ组，空白对照组；Ⅱ组，脂质体组；Ⅲ组（SCON），20 μmol/L正义寡核苷酸组；Ⅳ组（ASCON），20 μmol/L反义寡核苷酸组。上述各组均在培养24、36、48 h后，采用实时荧光定量方法检测VEGF及其受体Flt-1 mRNA表达情况的变化。［结果］VEGF mRNA在Ⅰ、Ⅱ组表达量无差异，具有较高的表达量，随着时间的延长无明显变化；在Ⅲ组VEGF mRNA 表达略有降低，但与Ⅰ和Ⅱ组间无显著性差异（P＞0.05）；Ⅳ组（反义寡核苷酸组）的VEGF mRNA表达量显著下降（P＜0.05），并且随着时间延长逐渐下降。VEGF受体FLT-1 mRNA的表达与VEGF相似。［结论］反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用。

Effects of Ghrelin Antisense Inhibition on VEGF and Its Receptor Fit-1 mRNA Expression

[ Objective ] This study was to investigate the effect of VEGF and its receptor Fh-1 mRNA expression in Mongolia sheep＇ s umbilical vein endothelial cells by ghrelin antisense inhibition. [ Method] Experiments were divided into 4 groups：group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ（SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fh-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [ Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱwere insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group Ⅰ and group Ⅱ （P 〉 0.05 ）, the expression of VEGF mRNA in group Ⅳ （ antisense oligonucleotide group ） decreased significantly （ P 〈 0.05 ） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF [ Conclusion ] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fh-1.