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| 一种改良的快速提取小型昆虫总RNA的方法 | |
| 引用本文： | 高阳,朱春晖,谭新球,刘勇,彭静,张德咏.一种改良的快速提取小型昆虫总RNA的方法[J].植物保护,2013,39(4):90-93. |
| 作者姓名： | 高阳 朱春晖 谭新球 刘勇 彭静 张德咏 |
| 作者单位： | 1. 湖南省植物保护研究所,长沙410125;中南大学研究生院隆平分院,长沙410125 2. 湖南省植物保护研究所,长沙410125;园艺作物病虫害治理湖南省重点实验室,长沙410125;中南大学研究生院隆平分院,长沙410125 3. 湖南省植物保护研究所,长沙410125;园艺作物病虫害治理湖南省重点实验室,长沙410125 |
| 基金项目： | 国家自然科学基金，国家大宗蔬菜产业技术体系 |
| 摘 要： | 硅粒吸附法是一种常用的植物总RNA提取方法,本文对其进行改良后应用于小型昆虫总RNA提取,同时与小型昆虫RNA提取常用的改进的一步法和Trizol法进行了比较.试验结果表明:改良的硅粒吸附法是一种经济高效的小型昆虫总RNA提取方法,RNA产率高,纯度佳.同时,整个试验操作步骤简单、省时(约1 h),所用试剂普遍、廉价. |
| 关 键 词： | 硅粒吸附法 总RNA提取 小型昆虫 |
A modified protocol for extraction of total RNA from small insects |
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| Gao Yang,Zhu Chunhui,Tan Xinqiu,Liu Yong,Peng Jing,Zhang Deyong.A modified protocol for extraction of total RNA from small insects[J].Plant Protection,2013,39(4):90-93. | |
| Authors: | Gao Yang Zhu Chunhui Tan Xinqiu Liu Yong Peng Jing Zhang Deyong |
| Institution: | 1. Hunan Institute of Plant Protection, Changsha410125, China; 2. Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province, Changsha410125, China; 3. Branch of Longping, Graduate School, Central South University, Changsha410125, China |
| Abstract: | The silica method, a simple and efficient protocol, is broadly used in plant RNA extraction. In this study, the method was modified and used in small insects, such as Myzus persicae, Bemisia tabaci Q sibling species and Frankliniella occidentalis. Meantime, we compared the RNA extraction efficiency of small insects with the modified silica protocol, the modified one step procedure and Trizol method. The results showed that the highest quality and yields of RNA were obtained by modified silica RNA extraction protocol. This protocol has the advantages of time saving, lower cost as well as easy to handle and can be used for RNA extraction of insects. |
| Keywords: | silica method total RNA extraction small insect |
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