硫酸安普霉素可溶性粉中非法添加乙酰甲喹的HPLC-PDA检测方法的建立

为检测硫酸安普霉素可溶性粉中非法添加的乙酰甲喹,以十八烷基硅烷键合硅胶为填充剂,磷酸盐缓冲液(取磷酸二氢钠3.0 g,加水1000 m L使溶解,加三乙胺0.5 m L,用饱和氢氧化钠溶液调节p H值至7.0)-甲醇为流动相,二极管阵列检测器(PDA),建立了HPLC-PDA检测方法,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。在此液相色谱条件下,乙酰甲喹与其他物质峰分离良好。按外标法以峰面积计算,乙酰甲喹的平均回收率为98.1%,RSD为0.3%。该检测方法简便、准确、可靠,可用于测定硫酸安普霉素可溶性粉中非法添加的乙酰甲喹。

Determination of Mequindox in Apramycin Sulfate Soluble Powder by HPLC-PDA

A method for the determination of mequindox in the apramycin sulfate soluble powder was developed by the high performance liquid chromatography with photo-diode array detector (HPLC-PDA). It was tested with C18 column, using phosphate buffer solution (taking the sodium dihydrogen phosphate 3.0 g, add 1000 mL water to dissolve, and add 0.5 mL triethylamine, adjusting pH to 7.0 by sodium hydroxide saturated solution) and methanol as the mobile phase. Peak purity test and spectrum similar test were helped to identify the mequindox. The mean recovery of mequindox was 98.1%, and RSD was 0.3%, respectively. In conclusion, the method is simple, accurate and reliable for the determination of mequindox in apramycin sulfate soluble powder.