| 牛早孕因子的分离纯化及其活性和含量测定 |
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| 引用本文： | 向际尧,李鹏程.牛早孕因子的分离纯化及其活性和含量测定[J].畜牧兽医学报,1995,26(2):132-133. |
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| 作者姓名： | 向际尧 李鹏程 |
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| 作者单位： | 中国科学院新疆化学研究所生化研究室 |
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| 摘 要： | 牛早孕因子的分离纯化及其活性和含量测定向际尧，李鹏程，孙文东，努尔波拉提（中国科学院新疆化学研究所生化研究室，乌鲁木齐８３００１１）早期妊娠因子（ＥａｒｌｙＰｒｅｇｎａｎｃｙＦａｃｔｏｒ，ＥＰＦ），由澳大利亚学者Ｈ。Ｍｏｒｔｏｎ等人在１９７４年应用玫...
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| 关 键 词： | 牛 早孕因子 EPF 分离纯化 活性检测 含量测定 |
THE ISOLATION-PURIFICATION OF COW EPF ANDDETECTION OF ITS ACTIVITY CONTENT |
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| Xiang Jiyao, Li Pengcheng, Sun Wendong, Nurbolat.THE ISOLATION-PURIFICATION OF COW EPF ANDDETECTION OF ITS ACTIVITY CONTENT[J].Acta Veterinaria et Zootechnica Sinica,1995,26(2):132-133. |
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| Authors: | Xiang Jiyao Li Pengcheng Sun Wendong Nurbolat |
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| Abstract: | EPF was isolated and purified from the blood of early pregnant cow using the tec-hniques of salting out, ion-exchange chromatograpliy, PAGE and electroelution etc. Itsactivity and content in blood was detected. The rate of E rosette forrning cell inhibitionwas 89.5%. SDS-PAGE analysis showed that the MW of the polypeptide was 44 KDa.the content of the EPF was about 1ug/dl in the blood. The polyclonal antibodies toEPF. Either in isolation or in combination with monoclonal antibodies to EPF, can beused in an early pregnancy diagnosis for mammalas, including humans in serum orurine. |
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| Keywords: | Pregnant cow Blood EPF |
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