二色胡枝子组织培养的研究

为探索建立植株再生系统技术,以二色胡枝子种子为材料,对该树种组织培养进行了研究.结果表明,以0.1%升汞对种子灭菌2 min,发芽率较高,污染率仅为5%;基础培养基为1/2 MS,直立苗比例达到80%以上,显著高于MS和B5培养基;在增殖培养中,BA对分化系数影响最大,NAA其次,ZT的作用不明显,适宜的增殖培养基为1/2 MS BA 1.0 mg/L NAA 0.01 mg/L,分化系数可达3.15;随着NAA浓度提高,对生根抑制作用加强,其浓度不宜超过0.5 mg/L, IBA对生根有促进作用,浓度以IBA 1.5 mg/L 为宜.

Studies on tissue culture of Lespedeza bicolor

Tissue culture of Lespedeza bicolor was studied in this experiment to develop a regeneration system.The results showed that the best disinfection time for Lespedeza bicolor seeds was 2 min with 0.1% HgCl_2,in which shooting percent reached 32.8%,while polluted percent of the seeds was just 5%.According to the results of experiment with orthogonal design,the concentration of 6-BA was the main factor which had a significant effect on the index of differentiation of buds.NAA was also effective as the second important factor and ZT seemed to have no effect.The optimum differentiation culture medium was 1/2 MS BA 1.0 mg/L NAA 0.01 mg/L,of which the index of generation could reach 3.15.The concentrations of NAA should be lower than 0.5 mg/L because inhibiting effect on rooting of plants became stronger along with its increasing.IBA had promoting effect on rooting of plants,and the optimum concentration was 1.5 mg/L.