| 陆地棉(G. hirsutum L.) N-乙酰氨基葡萄糖转移酶基因(GhGnT)的克隆及耐盐性功能分析 |
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| 引用本文： | 周凯,宋丽艳,王俊娟,王德龙,樊保香,王帅,叶武威.陆地棉(G. hirsutum L.) N-乙酰氨基葡萄糖转移酶基因(GhGnT)的克隆及耐盐性功能分析[J].分子植物育种,2011,9(3):309-317. |
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| 作者姓名： | 周凯 宋丽艳 王俊娟 王德龙 樊保香 王帅 叶武威 |
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| 作者单位： | 中国农业科学院棉花研究所棉花生物学国家重点实验室农业部棉花遗传改良重点实验室; |
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| 基金项目： | 973项目(2010CB126006); 转基因生物新品种培育科技重大专项(2008ZX08005)共同资助 |
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| 摘 要： | 在耐盐相关基因芯片基础上,我们筛选到盐诱导显著上调表达(Ratio>2)的N-乙酰氨基葡糖转移酶基因.选取耐盐材料中9806为材料,根据耐盐性抑制消减文库EST序列设计引物,利用RACE及RT-PCR技术获得该基因cDNA全长1970 by,命名为GhGnT.通过Blast比对,发现该基因与蓖麻乙酞氨基葡萄糖转移酶基因...
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| 关 键 词： | 基因芯片 乙酸氨基葡萄糖转移酶 Real-time 耐盐 陆地棉 |
Cloning and Salt-tolerance Analysis of N-acetylglucosaminyltransferase Gene (GhGnT) from Gossypium hirsutum L. |
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| Zhou Kai,Song Liyan,Wang Junjuan,Wang Delong,Fan Baoxiang,Wang Shuai,Ye Wuwei.Cloning and Salt-tolerance Analysis of N-acetylglucosaminyltransferase Gene (GhGnT) from Gossypium hirsutum L.[J].Molecular Plant Breeding,2011,9(3):309-317. |
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| Authors: | Zhou Kai Song Liyan Wang Junjuan Wang Delong Fan Baoxiang Wang Shuai Ye Wuwei |
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| Institution: | Zhou Kai Song Liyan Wang Junjuan Wang Delong Fan Baoxiang Wang Shuai Ye Wuwei Cotton Research Institute,Chinese Academy of Agricultural Science,State Key Laboratory of Cotton Biology,Key Laboratory of Cotton Genetic Improvement,MOA,Anyang,455000 |
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| Abstract: | Based on salt-tolerant gene chips, a N-acetylglucosaminyltransferase gene encoding resistance of salinity has been screened by ratio index (Ratio>2). Zhong9806, salt resistance spp. of Gossypium hirsutum L. was used as experimental materials, and primers were designed on the basis of EST from salt resistance related SSH library. The full cDNA, 1 970 bp long, was gained by using RACE and RT-PCR technology and the gene was named as GhGnT, which shows, by Blast comparison the highest simility, 82%, as the acet... |
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| Keywords: | Gene chips Acetylglucosaminyltransferase Real-time Salt tolerance Gossypium hirsutum L |
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