| 盐穗木耐盐基因通过花粉管通道法对棉花遗传转化的研究 |
| |
| 引用本文： | 王志才,廖茂森,木合热皮亚·艾尔肯,徐建辉,李春平,张富春.盐穗木耐盐基因通过花粉管通道法对棉花遗传转化的研究[J].分子植物育种,2011,9(2):180-184. |
| |
| 作者姓名： | 王志才 廖茂森 木合热皮亚·艾尔肯 徐建辉 李春平 张富春 |
| |
| 作者单位： | 1. 新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐,830046
2. 新疆农业科学院经济作物研究所,乌鲁木齐,830000 |
| |
| 基金项目： | 转基因生物新品种培育科技重大专项转基因棉花新品种培育课题(2009ZX08005-022B); 新疆维吾尔自治区科技重大专项(No200731138-3); 国家“973”计划前期研究专项(2009CB126006)共同资助 |
| |
| 摘 要： | 以新疆主栽陆地棉品种为实验材料,通过花粉管通道法导入盐穗木耐盐相关基因HcNHX1和HcVP1.经过连续两代筛选繁育,结果显示:外源基因已经整合进棉花受体材料基因组中,并且在RNA水平有表达:外源基因对T1代棉花转基因植株生长影响很大,表现为生长势弱,植株矮小,株铃少,但T2代的棉花转基因植株在株高、果枝数和株铃等方面...
|
| 关 键 词： | 耐盐基因 新疆主栽棉花 花粉管通道法 遗传转化 |
Study on Pollen-tube Pathway Mediated Cotton (Gossypium hirsutum) Transformation with Salt-tolerant Gene from Halostachys caspica |
| |
| Wang Zhicai,Liao Maosen,Muherepiya Aierken,Xu Jianhui,Li Chunping,Zhang Fuchun.Study on Pollen-tube Pathway Mediated Cotton (Gossypium hirsutum) Transformation with Salt-tolerant Gene from Halostachys caspica[J].Molecular Plant Breeding,2011,9(2):180-184. |
| |
| Authors: | Wang Zhicai Liao Maosen Muherepiya Aierken Xu Jianhui Li Chunping Zhang Fuchun |
| |
| Institution: | Wang Zhicai 1 Liao Maosen 1 Muherepiya Aierken 1 Xu Jianhui 2 Li Chunping 2 Zhang Fuchun 1* 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,830046,2 Institute of Industrial Crops,Xinjiang Academy of Agricultural Science,Xinjiang,830000 |
| |
| Abstract: | By using pollen-tube pathway method, the salt-tolerance relevant genes, HcNHX1 and HcVP1 from Halostachys caspica were introduced into Xinjiang upland cotton cultivars. Through two years of successive cultivation and screening, the main results were as follows: PCR test preliminarily showed that the foreign genes have been successfully integrated into cotton genome, and expressed as evidenced by RT-PCR assay. The integ- ration of foreign genes caused obvious variation of agronomic traits in T1 progenies, wh... |
| |
| Keywords: | Salt-tolerant gene Xinjiang upland cotton Pollen-tube pathway Genetic transformation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
